David Luminita, Danciu Virgil, Moldovan Bianca, Filip Adriana
Research Center for Advanced Chemical Analysis, Instrumentation and Chemometrics (ANALYTICA), Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, 11 Arany Janos Street, 400028 Cluj-Napoca, Romania.
Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, 1-3 Clinicilor Street, 400006 Cluj-Napoca, Romania.
Antioxidants (Basel). 2019 Apr 30;8(5):114. doi: 10.3390/antiox8050114.
Red fruits are considered a major source of antioxidant compounds in the human diet. They usually contain anthocyanins, phenolic pigments that confer them multiple health-promoting properties. The health benefits of these bioactive phytocompounds are strongly related to their bioavailability, which has been reported to be low. The aim of the present study is to investigate the changes in antioxidant capacity and anthocyanin content of Cornelian cherry fruit extract during gastrointestinal digestion. Thus, the work was designed using a simulated in vitro digestion model. The antioxidant capacity (AA) was tested by the 2,2-azinobis (3-ethylbenzothiazolyne-6-sulphonic acid) radical cation (ABTS) method, while quantification of anthocyanins (TAC) was accomplished by the means of the pH differential method and high performance liquid chromatography (HPLC). The results showed that gastric digestion had no significant effect on the TAC of the extract, while the AA slightly increased. After duodenal digestion, only 28.33% of TAC and 56.74% of AA were maintained. Cornelian cherries' anthocyanins were stable in stomach, so they can be absorbed in order to manifest their antioxidant capacity at the cellular level. The duodenal digestion dramatically decreased the TAC and AA level in the fruit extract.
红色水果被认为是人类饮食中抗氧化化合物的主要来源。它们通常含有花青素，这种酚类色素赋予它们多种促进健康的特性。这些生物活性植物化合物的健康益处与其生物利用度密切相关，据报道其生物利用度较低。本研究的目的是调查欧洲酸樱桃果实提取物在胃肠道消化过程中抗氧化能力和花青素含量的变化。因此，这项工作采用模拟体外消化模型进行设计。抗氧化能力（AA）通过2,2-联氮双（3-乙基苯并噻唑啉-6-磺酸）自由基阳离子（ABTS）法进行测试，而花青素定量（TAC）则通过pH差值法和高效液相色谱（HPLC）完成。结果表明，胃消化对提取物的TAC没有显著影响，而AA略有增加。十二指肠消化后，仅保留了28.33%的TAC和56.74%的AA。欧洲酸樱桃的花青素在胃中稳定，因此它们可以被吸收，以便在细胞水平上发挥其抗氧化能力。十二指肠消化显著降低了果实提取物中的TAC和AA水平。
