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在体外胃肠道消化条件下,菰(Rubus occidentalis L.)中花色苷的稳定性。

Stability of anthocyanins in bokbunja (Rubus occidentalis L.) under in vitro gastrointestinal digestion.

机构信息

Major of Food & Nutrition, Division of Applied Food System, Seoul Women's University, Seoul 01797, Republic of Korea.

Major of Food & Nutrition, Division of Applied Food System, Seoul Women's University, Seoul 01797, Republic of Korea.

出版信息

Food Chem. 2018 Nov 30;267:157-162. doi: 10.1016/j.foodchem.2018.02.109. Epub 2018 Feb 21.

DOI:10.1016/j.foodchem.2018.02.109
PMID:29934151
Abstract

The stability of anthocyanins in bokbunja (Rubus occidentalis L.) extract was investigated using an in vitro simulated gastrointestinal digestion. Ethanolic extract from bokbunja was digested with pepsin/HCl for 2 h at 37 °C, followed by pancreatin/bile salts for 2 h at 37 °C. Four anthocyanins including cyanidin-3-glucoside, cyanidin-3-sambubioside, cyanidin-3-xylosylrutinoside and cyanidin-rutinoside were identified in the bokbunja extract. The total anthocyanin content of bokbunja was 3.76 mg cyanidin-3-glucoside equivalents/g fresh weight (FW). Gastric digestion had no significant effect on anthocyanins. However, intestinal digestion substantially decreased anthocyanins up to 1.70 mg/g FW, corresponding to 45% of that in the bokbunja extract. This indicates that about half of anthocyanins can reach an intestinal tract. In addition, a new compound comprised of cyanidin-3-glucosdie, catechin and acetaldehyde in a separated study was observed after in vitro gastrointestinal digestion. This shows that anthocyanins could be transformed into other compounds with different biochemical properties.

摘要

采用体外模拟胃肠道消化法研究了桑果(Rubus occidentalis L.)提取物中花色苷的稳定性。用胃蛋白酶/盐酸在 37°C 下消化桑果的乙醇提取物 2 h,然后用胰酶/胆汁盐在 37°C 下消化 2 h。在桑果提取物中鉴定出 4 种花色苷,包括矢车菊素-3-葡萄糖苷、矢车菊素-3-山梨糖苷、矢车菊素-3-木糖苷和矢车菊素-芦丁糖苷。桑果中总花色苷含量为 3.76mg 矢车菊素-3-葡萄糖苷当量/g 鲜重(FW)。胃消化对花色苷没有显著影响。然而,肠消化会使花色苷大大减少,至 1.70mg/g FW,相当于桑果提取物中花色苷含量的 45%。这表明约一半的花色苷可以到达肠道。此外,在另一项体外胃肠道消化研究中观察到一种由矢车菊素-3-葡萄糖苷、儿茶素和乙醛组成的新化合物。这表明花色苷可能转化为具有不同生化特性的其他化合物。

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