Berencsi G, Dyachenko N S, Tarassishin L A, Vantsak N P, Kovalishin G G, Kiseleva E K, Zhovnovataya V Z, Adám E, Nász I
Acta Microbiol Hung. 1986;33(3):233-43.
Two descendants of the prototype strain AD71-Washington D. C. were obtained by independent passaging for at least 18 years in Kiev, and in Budapest (Ad h 1 kappa, and Ad h 1B, respectively). By restriction endonuclease mapping, the DNA was identical corresponding to the patterns of human adenovirus type 1. In spite of this, SDS-polyacrylamide gel electrophoresis revealed that the purified hexon of Ad h 1 kappa was of lower Mr than the subunit of Ad h 1B. In contrast to this, the native capsomer (hexon) of Ad h 1 kappa exhibited lower electrophoretic mobility in agarose gel electrophoresis than the native hexon of Ad h 1B. Oligopeptide mapping of the main hexon bands from SDS-polyacrylamide gels revealed the presence of unique spots among the chymotryptic oligopeptides of Ad h 1B, too. Thus, the differences in the sensitivity to proteolytic cleavage during purification seem to have a structural basis. Antigenic analysis of the native hexon capsomers was performed using polyclonal antihexon immunsera. Immunodiffusion, immunoelectrophoresis, and competitive RIA were used for comparison. The results indicate that native hexon capsomers of Ad h 1 kappa and Ad ha 1B possess antigenic differences within the type-specific regions, nevertheless, their genetic background could not be detected by the restriction endonucleases applied. It cannot be excluded that the differences were results of altered assembly of virions under different passage conditions.
原型菌株AD71 - 华盛顿特区的两个后代分别在基辅和布达佩斯通过至少18年的独立传代获得（分别为Ad h 1 κ和Ad h 1B）。通过限制性内切酶图谱分析，其DNA与1型人腺病毒的图谱相同。尽管如此，SDS - 聚丙烯酰胺凝胶电泳显示，纯化的Ad h 1 κ六邻体的Mr低于Ad h 1B的亚基。与此相反，Ad h 1 κ的天然衣壳粒（六邻体）在琼脂糖凝胶电泳中的电泳迁移率低于Ad h 1B的天然六邻体。对SDS - 聚丙烯酰胺凝胶上主要六邻体条带的寡肽图谱分析表明，在Ad h 1B的胰凝乳蛋白酶寡肽中也存在独特的斑点。因此，纯化过程中对蛋白水解切割敏感性的差异似乎有结构基础。使用多克隆抗六邻体免疫血清对天然六邻体衣壳粒进行抗原分析。采用免疫扩散、免疫电泳和竞争性放射免疫分析进行比较。结果表明，Ad h 1 κ和Ad h 1B的天然六邻体衣壳粒在型特异性区域内存在抗原差异，然而，应用的限制性内切酶未能检测到它们的遗传背景。不能排除这些差异是不同传代条件下病毒粒子组装改变的结果。
