基于离子液体的超声波辅助萃取结合 HPLC 分析七种化合物。
Ionic Liquid-Based Ultrasonic-Assisted Extraction to Analyze Seven Compounds in Coupled with HPLC.
机构信息
National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China.
Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Kaifeng 475004, China.
出版信息
Molecules. 2019 Apr 30;24(9):1699. doi: 10.3390/molecules24091699.
is widely used in traditional Chinese medicine (TCM), and the content of psoralen, isopsoralen, neobavaisoflavone, bavachin, psoralidin, isobavachalcone, and bavachinin A is the main quality control index of because of its clinical effects. Thus, a fast and environmentally-benign extraction method of seven compounds in is necessary. In this work, an ionic liquid-based ultrasonic-assisted method (ILUAE) for the extraction of seven compounds from was proposed. Several ILs of different types and parameters, including the concentration of ILs, concentration of ethanol (EtOH), solid-liquid ratio, particle size, ultrasonic time, centrifugal speed, and ultrasonic power, were optimized by the Placket-Burman (PB) design and Box-Behnken response surface analysis. Under this optimal condition, the total extraction yield of the seven compounds in was 18.90 mg/g, and significantly greater than the conventional 75% EtOH solvent extraction.
广泛应用于中药（TCM），其补骨脂素、异补骨脂素、新补骨脂异黄酮、补骨脂甲素、补骨脂定、异补骨脂查尔酮和补骨脂宁 A 的含量是 的主要质量控制指标。因此，需要一种快速、环境友好的七种化合物的提取方法。在这项工作中，提出了一种基于离子液体的超声辅助法（ILUAE）从 中提取七种化合物。通过 Placket-Burman（PB）设计和 Box-Behnken 响应面分析，对不同类型和参数的几种离子液体，包括离子液体浓度、乙醇（EtOH）浓度、固液比、粒径、超声时间、离心速度和超声功率进行了优化。在最佳条件下， 的七种化合物的总提取率为 18.90mg/g，明显高于常规的 75%EtOH 溶剂提取。
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