The MOE Key Laboratory for Standardization of Chinese Medicines and The Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.
Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai, People's Republic of China.
J Sep Sci. 2017 Nov;40(21):4191-4202. doi: 10.1002/jssc.201700482. Epub 2017 Sep 26.
Psoraleae Fructus is one of the most popular traditional Chinese medicines. Coumarins, flavonoids, and meroterpenes are the main contributors to the biological activity of Psoraleae Fructus. In this study, a new method for the quality control of Psoraleae Fructus was developed, through the quantitative analysis of multicomponents by single marker with diode array detector. Thirteen components, including psoralenoside, isopsoralenoside, psoralen, isopsoralen, psoralidin, neobavaisoflavone, bavachin, corylin, isobavachalcone, corylifol A, bavachinin, bavachalcone, and bakuchiol were rapidly separated and identified within 12 min by the newly developed method. The feasibility and reliability of this method were corroborated. The method was also compared to the external standard method and detection by corona charged aerosol detector. The results of percent difference (%) and cos (θ) have shown that there were no significant differences observed between the quantitative analysis of multicomponents by single marker and external standard method analyses; psoralen and isopsoralen were undetectable with the corona charged aerosol detector due to their but the sensitivity for all the compounds except bakuchiol detected by corona charged aerosol detector are higher than those obtained by diode array detector. In addition, the newly method developed was applied to the quality evaluation of Chinese patent medicines containing Psoraleae Fructus.
补骨脂为常用中药之一，香豆素、黄酮、苯并呋喃二萜类化合物是其发挥生物活性的主要物质基础。本研究建立了一种以二极管阵列检测器为检测手段，以单标志物定量测定多组分的补骨脂药材质量控制新方法。该方法在 12 min 内实现了 13 种成分（补骨脂素、异补骨脂素、补骨脂甲素、异补骨脂甲素、补骨脂乙素、新异补骨脂二氢黄酮、补骨脂查尔酮、补骨脂甲素、异补骨脂甲素、异补骨脂查尔酮、补骨脂定、补骨脂乙素、补骨脂二氢黄酮甲醚和补骨脂醇）的快速分离与鉴定。验证了该方法的可行性和可靠性，并与外标法和荷电气溶胶检测法进行了比较。结果表明，单标志物定量法与外标法之间无显著差异；由于荷电气溶胶检测的灵敏度较低，补骨脂素和异补骨脂素未能检出，但除补骨脂醇外，所有化合物的检测灵敏度均高于二极管阵列检测器。此外，该方法还用于含补骨脂的中成药的质量评价。
