de Hemptinne A
Pflugers Arch. 1978 Nov 30;377(3):235-43. doi: 10.1007/BF00584278.
Steady state and non steady state I-V relationships of the resting membrane and delayed rectifying membrane were analysed by applying trapezoid voltage clamp pulses on atrial fibres isolated from the frog and the tortoise. The fibres were disposed in a perfusion chamber for double sucrose gap. In both types of preparation, a component of delayed rectification current was identified which was activated following a comparable time course. The amplitude of the delayed rectification current, when expressed either as normalized to the calculated membrane capacity or to the initial background current, is significantly larger in the frog than in the tortoise. Full activation of the delayed rectifying system can be demonstrated in the tortoise, while in the frog this process is presumably complicated by simultaneous accumulation of K ions in the extracellular region. The difference in magnitude of the delayed outward current has no influence on the duration of action potential which is recorded over the sucrose gap.
通过对从青蛙和乌龟分离出的心房纤维施加梯形电压钳脉冲，分析了静息膜和延迟整流膜的稳态和非稳态电流-电压关系。将纤维置于用于双蔗糖间隙的灌注室中。在这两种制备类型中，均鉴定出延迟整流电流的一个成分，其在相当的时间进程后被激活。当以相对于计算出的膜电容或初始背景电流进行归一化表示时，延迟整流电流的幅度在青蛙中明显大于乌龟。在乌龟中可以证明延迟整流系统的完全激活，而在青蛙中，这个过程可能因细胞外区域钾离子的同时积累而变得复杂。延迟外向电流大小的差异对通过蔗糖间隙记录的动作电位持续时间没有影响。
