The Key Laboratory of Mariculture (Ministry of Education), The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture), Fisheries College, Ocean University of China, Qingdao, 266003, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Wen Hai Road, Qingdao, 266237, China.
Fish Physiol Biochem. 2019 Oct;45(5):1603-1614. doi: 10.1007/s10695-019-00646-1. Epub 2019 May 3.
In order to study the effects of dietary fatty acid compositions on glucose metabolism, large yellow croaker juveniles Larimichthys crocea (initial weight, 36.80 ± 0.39 g) were fed with two experiment diets for 12 weeks. The two diets contained 6.5% of fish oil (FO) and palm oil (PO), respectively. Results showed that the contents of saturated fatty acids in liver and muscle, levels of glucose, triglyceride (TG), non-esterified fatty acid (NEFA), and leptin in blood were significantly higher in PO group, while the hepatic glycogen and muscle glycogen significantly decreased (P < 0.05). There were no significant differences in blood insulin and adiponectin levels between the two groups (P > 0.05). Compared with the FO group, the expressions of glucokinase (GK), glucose-6-phosphate dehydrogenase, glycogen synthase (GYS), glucose transporter 2 (GLUT2), insulin receptor 1 (IR1), insulin receptor substrate 1 (IRS1), insulin receptor substrate (IRS2), and protein kinase B (AKT2) were significantly decreased, and the expressions of phosphoenolpyruvate carboxykinase (PEPCK) in liver were significantly increased in the PO group. Meanwhile, the expressions of GK, phosphofructokinase, GYS, GLUT4, and insulin receptor 2 (IR2) were significantly reduced, and the expressions PEPCK, fructose-1 and 6-diphosphatase in muscle were significantly increased in the PO group. In conclusion, palm oil in diet could inhibit the utilization of glucose and promote the endogenous glucose production in large yellow croaker by reducing the sensitivity of insulin, so as to increase the blood glucose level.
为了研究膳食脂肪酸组成对葡萄糖代谢的影响，用两种实验饲料喂养大黄鱼幼鱼（初始体重 36.80±0.39 g）12 周。两种饲料分别含有 6.5%的鱼油（FO）和棕榈油（PO）。结果表明，PO 组肝、肌肉中饱和脂肪酸含量、血糖、甘油三酯（TG）、非酯化脂肪酸（NEFA）和瘦素水平显著升高，肝糖原和肌肉糖原含量显著降低（P<0.05）。两组间血液胰岛素和脂联素水平无显著差异（P>0.05）。与 FO 组相比，PO 组肝葡萄糖激酶（GK）、葡萄糖-6-磷酸脱氢酶、糖原合酶（GYS）、葡萄糖转运蛋白 2（GLUT2）、胰岛素受体 1（IR1）、胰岛素受体底物 1（IRS1）、胰岛素受体底物 2（IRS2）和蛋白激酶 B（AKT2）的表达显著降低，肝磷酸烯醇丙酮酸羧激酶（PEPCK）的表达显著升高。同时，PO 组肝磷酸果糖激酶、GYS、GLUT4 和胰岛素受体 2（IR2）的表达显著降低，肌肉中 PEPCK、果糖-1,6-二磷酸酶的表达显著升高。综上所述，饲料中的棕榈油通过降低胰岛素的敏感性，抑制大黄鱼对葡萄糖的利用，促进内源性葡萄糖产生，从而提高血糖水平。
