高比例膳食棕榈油通过激活 TLR-NF-κB 信号通路抑制大黄鱼生长和抗氧化能力并诱导炎症。
High percentage of dietary palm oil suppressed growth and antioxidant capacity and induced the inflammation by activation of TLR-NF-κB signaling pathway in large yellow croaker (Larimichthys crocea).
机构信息
Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture, The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 5 Yushan Road, Qingdao, Shandong, 266003, People's Republic of China.
Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture, The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 5 Yushan Road, Qingdao, Shandong, 266003, People's Republic of China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong, 266237, People's Republic of China.
出版信息
Fish Shellfish Immunol. 2019 Apr;87:600-608. doi: 10.1016/j.fsi.2019.01.055. Epub 2019 Feb 6.
A 70-day feeding trial was conducted to investigate the effects of dietary fish oil (FO) replaced by palm oil (PO) on growth, biochemical and antioxidant response as well as inflammatory response in the liver of large yellow croaker (initial weight 15.87 ± 0.14 g). Four iso-proteic and iso-lipidic experimental diets were formulated with 0% (the control group), 33.3%, 66.7% and 100% FO replaced by PO. Fish fed the diet with 100% PO showed significantly lower growth performance than the control group. As expected, the contents of C16:0, C18:1n-9 and C18:2n-6 were increased with increasing dietary PO levels. There were remarkable increases in total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) levels in fish fed the diet with 100% PO compared to the control group. Moreover, dietary PO significantly increased activities of plasma alanine transaminase (ALT) and aspartate aminotransferase (AST) in fish fed the diet with 100% PO compared to the control group. The total antioxidant capacity (T-AOC) and the activity of catalase (CAT) in plasma were significantly decreased in fish fed the diet with 100% PO compared to the control group, and meanwhile no significant differences were found in T-AOC and CAT activity in fish fed diets with no more than 66.7% PO. Fish fed the diet with 100% PO exerted significantly higher toll like receptors (TLRs) and myeloid differentiation factor (MyD88) mRNA expression levels than the control group. The IFNγ, IL-1β and TNFα mRNA expressions were increased with increasing dietary PO levels. The increase of pro-inflammatory gene expression may be due to the activation of NF-κB signaling as the ratio of nucleus p65 to total p65 protein was elevated with the increase of dietary PO levels. These results showed that relatively higher PO levels in diets suppressed the growth and antioxidant capacity as well as induced the inflammatory response by activating TLR-NF-κB signaling pathway in juvenile large yellow croaker.
进行了一项为期 70 天的饲养试验，研究了用棕榈油（PO）替代日粮中的鱼油（FO）对大黄鱼（初始体重 15.87±0.14g）生长、生化和抗氧化反应以及肝脏炎症反应的影响。用 0%（对照组）、33.3%、66.7%和 100%FO 替代的四种等蛋白和等脂的实验饲料进行了配方设计。与对照组相比，摄食 100%PO 饲料的鱼的生长性能显著降低。正如预期的那样，随着日粮 PO 水平的增加，C16:0、C18:1n-9 和 C18:2n-6 的含量增加。与对照组相比，摄食 100%PO 饲料的鱼的总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）水平显著升高。此外，与对照组相比，摄食 100%PO 饲料的鱼的血浆丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）活性显著升高。与对照组相比，摄食 100%PO 饲料的鱼的血浆总抗氧化能力（T-AOC）和过氧化氢酶（CAT）活性显著降低，而摄食不超过 66.7%PO 的鱼的 T-AOC 和 CAT 活性没有差异。摄食 100%PO 饲料的鱼的 toll 样受体（TLRs）和髓样分化因子（MyD88）mRNA 表达水平显著高于对照组。随着日粮 PO 水平的增加，IFNγ、IL-1β和 TNFα mRNA 的表达增加。促炎基因表达的增加可能是由于 NF-κB 信号通路的激活，因为随着日粮 PO 水平的增加，核 p65 与总 p65 蛋白的比值升高。这些结果表明，饲料中较高的 PO 水平通过激活 TLR-NF-κB 信号通路，抑制了幼大黄鱼的生长和抗氧化能力，并诱导了炎症反应。
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