高水平的膳食橄榄油通过激活大黄花鱼（Larimichthys crocea）的 p38 MAPK 和 JNK 通路，降低了生长速度，增加了肝脏脂质沉积，并引发了炎症。
High level of dietary olive oil decreased growth, increased liver lipid deposition and induced inflammation by activating the p38 MAPK and JNK pathways in large yellow croaker (Larimichthys crocea).
机构信息
Key laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture and Rural Affairs, and the key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 5 Yushan Road, Qingdao, Shandong, 266003, People's Republic of China.
Key laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture and Rural Affairs, and the key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 5 Yushan Road, Qingdao, Shandong, 266003, People's Republic of China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong, 266237, People's Republic of China.
出版信息
Fish Shellfish Immunol. 2019 Nov;94:157-165. doi: 10.1016/j.fsi.2019.08.062. Epub 2019 Aug 26.
A feeding experiment was conducted to determine the effects of fish oil replaced by olive oil (OO) on growth performance, serum biochemical, antioxidant capacity and inflammatory response in large yellow croaker (Larimichthys crocea). Four iso-nitrogenous and iso-lipidic diets were formulated by replacing fish oil (FO) with 0% (the control group), 33.3%, 66.7% and 100% OO. Fish fed the diet with 100% OO had the lowest growth performance among dietary treatments. However, there were no significant differences in SGR and FI among fish fed diets with 0% (the control group), 33.3% and 66.7% OO (P > 0.05). As to morphological parameters, HSI was significantly increased in fish fed the diet with 100% OO than the control group (P < 0.05). Furthermore, the lipid content of the liver in fish fed the diet with 100% OO was significantly higher than the control group (P < 0.05). Fish fed the diet with 100% OO had the highest content of C18:1n-9 among dietary treatments. Serum total triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C) levels and activity of serum alanine transaminase (ALT) were significantly increased in fish fed the diet with 100% OO compared with the control group (P < 0.05). Meanwhile, dietary OO decreased the activity of superoxide dismutase (SOD) and the total antioxidant capacity (T-AOC) in fish fed diets with increasing dietary OO levels. However, the content of malondialdehyde (MDA) was significantly increased in fish fed the diet with 100% OO compared with the control group (P < 0.05). The expression of pro-inflammatory genes, COX-2, IL-1β and TNFα, were significantly increased in the liver of fish fed the diet with 100% OO compared with the control group (P < 0.05), which was probably due to the activation of p38 mitogen-activated protein kinase (p38 MAPK) pathways and Jun N-terminal kinase (JNK) as the increased protein ratio of p-p38 MAPK to p38 MAPK and p-JNK to JNK. These results suggested that high level of dietary OO decreased the growth performance and antioxidant capacity but induced inflammation via the activation of p38 MAPK and JNK pathways in large yellow croaker.
进行了一项摄食实验，以确定用橄榄油（OO）替代鱼油对大黄鱼（Larimichthys crocea）生长性能、血清生化、抗氧化能力和炎症反应的影响。通过用 0%（对照组）、33.3%、66.7%和 100%OO 替代鱼油，配制了四种等氮等脂的饲料。摄食 100%OO 饲料的鱼在饲养处理中生长性能最低。然而，摄食 0%（对照组）、33.3%和 66.7%OO 饲料的鱼的 SGR 和 FI 没有显著差异（P>0.05）。至于形态参数，摄食 100%OO 饲料的鱼的 HSI 显著高于对照组（P<0.05）。此外，摄食 100%OO 饲料的鱼的肝脏脂质含量显著高于对照组（P<0.05）。摄食 100%OO 饲料的鱼的 C18:1n-9 含量最高。与对照组相比，摄食 100%OO 饲料的鱼的血清总甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）水平和血清丙氨酸转氨酶（ALT）活性显著升高（P<0.05）。同时，随着饲料中 OO 含量的增加，饲料中的 OO 降低了摄食鱼类的超氧化物歧化酶（SOD）和总抗氧化能力（T-AOC）的活性。然而，摄食 100%OO 饲料的鱼的丙二醛（MDA）含量显著高于对照组（P<0.05）。与对照组相比，摄食 100%OO 饲料的鱼的肝脏中促炎基因 COX-2、IL-1β 和 TNFα 的表达显著升高（P<0.05），这可能是由于 p38 丝裂原活化蛋白激酶（p38 MAPK）途径和 Jun N-末端激酶（JNK）的激活，导致 p-p38 MAPK 与 p38 MAPK 和 p-JNK 与 JNK 的蛋白比增加。这些结果表明，大黄鱼饲料中高水平的 OO 通过激活 p38 MAPK 和 JNK 途径，降低了生长性能和抗氧化能力，但诱导了炎症。
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