Atal Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Port Blair, 744103, Andaman and Nicobar Islands, India.
Marine Biotechnology Division, Ocean Science and Technology for Islands Group, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Chennai, 600100, Tamil Nadu, India.
Microb Pathog. 2019 Jul;132:129-136. doi: 10.1016/j.micpath.2019.04.043. Epub 2019 May 1.
Deep Sea sediment cores were collected from the surrounding of active volcanic Barren Island, Andaman & Nicobar Islands. A total of 123 cultivable marine actinobacteria were isolated and identified based on their biochemical and 16S rDNA sequences. The isolates were categorized under 10 genera, of which Streptomyces sp., Dietzia sp. and Brevibacterium sp. are the dominant genera. Of 123 isolates, 73 isolates exhibited appreciable inhibitory activity against both Gram positive and Gram negative bacteria. High frequencies of positive PCR amplification were obtained for PKS-I (39.13%), PKS-II (56.52%) and NRPS (69.57%). The highest levels of biosynthetic systems were observed for NRPS and PKS-II. Majority of the actinobacterial isolates revealed excellent potential for bioprospecting of novel byproducts with industrial and pharmaceutical importance.
从安得拉邦和尼科巴群岛的巴伦岛周围的活动火山采集了深海沉积物岩芯。根据生化和 16S rDNA 序列，共分离和鉴定了 123 株可培养海洋放线菌。这些分离物分为 10 个属，其中链霉菌属、地衣芽孢杆菌属和短杆菌属是主要属。在 123 株分离物中，有 73 株对革兰氏阳性菌和革兰氏阴性菌均表现出明显的抑制活性。PKS-I（39.13%）、PKS-II（56.52%）和 NRPS（69.57%）的 PCR 扩增阳性率较高。NRPS 和 PKS-II 的生物合成系统水平最高。大多数放线菌分离株显示出具有工业和制药重要性的新型副产物的生物勘探的巨大潜力。
