Department of Marine Science, College of Natural Sciences, Incheon National University, Incheon 22012, South Korea.
Department of Marine Science, College of Natural Sciences, Incheon National University, Incheon 22012, South Korea; Research Institute of Basic Sciences, Incheon National University, Incheon 22012, South Korea.
Comp Biochem Physiol C Toxicol Pharmacol. 2019 Aug;222:125-134. doi: 10.1016/j.cbpc.2019.05.001. Epub 2019 May 2.
Sea-Nine™ 211 is an emerging biocide that has an adverse impact on aquatic environments. In this study, the marine polychaete Perinereis aibuhitensis was exposed to Sea-Nine (0.1, 1, and 10 μg L), and acute toxicity and biochemical responses such as changes in the intracellular contents of malondialdehyde (MDA) and glutathione (GSH) and enzymatic activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST), and acetylcholinesterase (AChE) were evaluated over a period of 14 d. Determined median lethal doses, LC50 were 268 μg L, 142 μg L, and 55 μg L at 24 h, 96 h, and 14 d, respectively. The MDA content increased significantly in a dose- and time-dependent manner, indicative of lipid peroxidation-related oxidative damage. Significantly higher intracellular GSH levels and antioxidant defense-related enzyme (CAT, SOD, GPx, GR, and GST) activities were observed after exposure to 10 μg L Sea-Nine. In contrast, Sea-Nine treatment significantly reduced AChE activity at the highest concentration of Sea-Nine used (10 μg L). Taken together, these results indicate that sublethal concentrations of Sea-Nine are toxic to marine polychaetes through potential lipid peroxidation, induction of oxidative stress, and modulation of the cholinergic system. Our results can contribute to biomonitoring of aquatic environments and ecotoxicological research through the measurements of polychaete cellular defenses against waterborne biocides.
海Nine™211 是一种新兴的杀生物剂，对水生环境有不利影响。在这项研究中，海洋多毛类动物沙蚕 Perinereis aibuhitensis 暴露于海 Nine（0.1、1 和 10μg/L）中，评估了急性毒性和生化反应，如丙二醛（MDA）和谷胱甘肽（GSH）的细胞内含量变化以及过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）、谷胱甘肽 S-转移酶（GST）和乙酰胆碱酯酶（AChE）的酶活性，为期 14 天。在 24 h、96 h 和 14 d 时，确定的中致死剂量 LC50 分别为 268μg/L、142μg/L 和 55μg/L。MDA 含量呈剂量和时间依赖性显著增加，表明与脂质过氧化相关的氧化损伤。暴露于 10μg/L 海 Nine 后，细胞内 GSH 水平和抗氧化防御相关酶（CAT、SOD、GPx、GR 和 GST）活性显著升高。相反，在使用的最高海 Nine 浓度（10μg/L）下，海 Nine 处理显著降低了 AChE 活性。综上所述，这些结果表明，亚致死浓度的海 Nine 通过潜在的脂质过氧化、氧化应激诱导和胆碱能系统的调节对海洋多毛类动物有毒。我们的研究结果可以通过测量多毛类动物对水生物杀菌剂的细胞防御来为水生环境的生物监测和生态毒理学研究做出贡献。
