Faculdade de Ciências e Tecnologia (FCT)-Universidade Estadual Paulista (UNESP)-Presidente Prudente, SP 19060-900, Brazil.
Faculdade de Ciências e Tecnologia (FCT)-Universidade Estadual Paulista (UNESP)-Presidente Prudente, SP 19060-900, Brazil.
Biophys Chem. 2019 Jul;250:106176. doi: 10.1016/j.bpc.2019.106176. Epub 2019 Apr 26.
Giant unilamellar vesicles (GUVs) have been one of the most extensively investigated membrane model to study cell membrane-ligand interactions. In this study, we investigated the interaction between glyphosate and picloram with GUVs made with sphingomyelin (SM), cholesterol (CHOL), and dioleoyl-sn-glycerol-3-phosphocholine (DOPC) (DOPC/SM (1:1), DOPC/CHOL/SM (1:1:1)) in a physiological environment using confocal and phase contrast microscopy. At high pesticide concentrations (70 to 90 μM), we generally found the GUVs undergoing a physical such as contouring, elongation, and eventually lose their characteristic spherical shape. In addition, to determine the comparative effect of the pesticides, control experiments were performed using GUVs made with only DOPC and DOPC/SM 1:1. The results show that, at low concentration (0.5 μM), a significant effect was observed during a 30 min incubation time. These findings also suggest that cholesterol may play a significant role in the permeability of the vesicle against the action of the pesticides, which have important biological implications on the lipid composition of the membrane.
巨大单层囊泡（GUVs）是研究细胞膜-配体相互作用的最广泛研究的膜模型之一。在这项研究中，我们使用含有神经鞘磷脂（SM）、胆固醇（CHOL）和二油酰基-sn-甘油-3-磷酸胆碱（DOPC）的 GUVs（DOPC/SM（1:1）、DOPC/CHOL/SM（1:1:1））在生理环境中使用共聚焦和相差显微镜研究草甘膦和草铵膦与 GUVs 的相互作用。在高农药浓度（70 至 90 μM）下，我们通常发现 GUVs 经历物理变化，例如轮廓变形、伸长，最终失去其特征的球形形状。此外，为了确定农药的比较效果，使用仅由 DOPC 和 DOPC/SM 1:1 制成的 GUVs 进行了对照实验。结果表明，在低浓度（0.5 μM）下，在 30 分钟孵育时间内观察到显著效果。这些发现还表明胆固醇可能在囊泡对农药作用的通透性中起重要作用，这对膜的脂质组成具有重要的生物学意义。
