García Cánovas F, Tudela J, Martínez Madrid C, Varón R, García Carmona F, Lozano J A
Biochim Biophys Acta. 1987 Apr 30;912(3):417-23. doi: 10.1016/0167-4838(87)90047-1.
Tyrosinase has a suicide inactivation reaction when it acts on omicron-diphenols. In the present paper, this reaction has been studied using a transient phase approach. Explicit equations of product vs. time have been developed for the multisubstrate mechanism of tyrosinase, and the kinetic parameters which characterize the enzyme acting on the suicide substrate catechol have been determined. The effect of pH has also been considered.
酪氨酸酶作用于邻二酚时会发生自杀失活反应。在本文中，采用瞬态相方法对该反应进行了研究。针对酪氨酸酶的多底物机制，推导了产物随时间变化的显式方程，并确定了表征该酶作用于自杀底物儿茶酚的动力学参数。同时还考虑了pH的影响。
