Fukuta Yasuhisa, Kamei Kengo, Matsui Aoi, Fuji Yosuke, Onuma Hiroki, Shirasaka Norifumi
a Department of Applied Biological Chemistry, Faculty of Agriculture , Kindai University , Nara , Japan.
Biosci Biotechnol Biochem. 2019 Jul;83(7):1354-1361. doi: 10.1080/09168451.2019.1611406. Epub 2019 May 6.
is a pink mushroom. This pink color is a protein and forms a complex with 3H-indol-3-one. The gene encoding the pink-colored protein from (PsPCP) was cloned, and its sequence was elucidated as a 681-bp. The ORF encodes 226 amino acid residues. The amino acid sequence of the protein did not show any significant homology in the DDBJ/EMBL/GenBank databases. Recombinant PsPCP was expressed as the soluble form in . The reaction mixture of purified recombinant PsPCP and 3H-indol-3-one showed a pink color as the native pigment. A real-time PCR analysis revealed the strong expression of PsPCP in the primordium formation stage of the life cycle of the fungus; however, its expression decreased with the maturation of the fruit body. A comparison of PsPCP gene expression profiles between two strains revealed high levels in the dark-colored strain.
是一种粉红色蘑菇。这种粉红色是一种蛋白质，它与3H-吲哚-3-酮形成复合物。克隆了来自（PsPCP）的编码粉红色蛋白质的基因，其序列被阐明为681个碱基对。开放阅读框编码226个氨基酸残基。该蛋白质的氨基酸序列在DDBJ/EMBL/GenBank数据库中未显示出任何显著的同源性。重组PsPCP以可溶形式在中表达。纯化的重组PsPCP与3H-吲哚-3-酮的反应混合物呈现出与天然色素相同的粉红色。实时PCR分析显示，PsPCP在真菌生命周期的原基形成阶段强烈表达；然而，随着子实体的成熟，其表达下降。两个菌株之间PsPCP基因表达谱的比较显示深色菌株中的表达水平较高。
