Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, People's Republic of China.
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, People's Republic of China.
Genomics. 2018 May;110(3):201-209. doi: 10.1016/j.ygeno.2017.09.012. Epub 2017 Sep 29.
Blue light is an important environmental factor which could induce mushroom primordium differentiation and fruiting body development. However, the mechanisms of Pleurotus eryngii primordium differentiation and development induced by blue light are still unclear. The CAZymes (carbohydrate-active enzymes) play important roles in degradation of renewable lignocelluloses to provide carbohydrates for fungal growth, development and reproduction. In the present research, the expression profiles of genes were measured by comparison between the Pleurotus eryngii at primordium differentiated into fruiting body stage after blue light stimulation and dark using high-throughput sequencing approach. After assembly and compared to the Pleurotus eryngii reference genome, 11,343 unigenes were identified. 539 differentially expressed genes including white collar 2 type of transcription factor gene, A mating type protein gene, MAP kinase gene, oxidative phosphorylation associated genes, CAZymes genes and other metabolism related genes were identified during primordium differentiated into fruiting body stage after blue light stimulation. KEGG results showed that carbon metabolism, glycolysis/gluconeogenesis and biosynthesis of amino acids pathways were affected during blue light inducing primordia formation. Most importantly, 319 differentially expressed CAZymes participated in carbon metabolism were identified. The expression patterns of six representative CAZymes and laccase genes were further confirmed by qRT-PCR. Enzyme activity results indicated that the activities of CAZymes and laccase were affected in primordium differentiated into fruiting body under blue light stimulation. In conclusion, the comprehensive transcriptome and CAZymes of Pleurotus eryngii at primordium differentiated into fruiting body stage after blue light stimulation were obtained. The biological insights gained from this integrative system represent a valuable resource for future genomic studies on this commercially important mushroom.
蓝光是一种重要的环境因素，可诱导蕈菌原基分化和子实体发育。然而，蓝光诱导杏鲍菇原基分化和发育的机制尚不清楚。CAZymes（碳水化合物活性酶）在可再生木质纤维素的降解中发挥重要作用，为真菌的生长、发育和繁殖提供碳水化合物。在本研究中，通过高通量测序方法比较蓝光刺激后杏鲍菇原基分化为子实体阶段和黑暗条件下的基因表达谱，来测量基因的表达谱。经过组装并与杏鲍菇参考基因组进行比较，鉴定出 11343 个 unigenes。鉴定出 539 个差异表达基因，包括白环 2 型转录因子基因、A 交配型蛋白基因、MAP 激酶基因、氧化磷酸化相关基因、CAZymes 基因和其他代谢相关基因，这些基因在蓝光刺激后原基分化为子实体阶段。KEGG 结果表明，碳代谢、糖酵解/糖异生和氨基酸生物合成途径在蓝光诱导原基形成过程中受到影响。最重要的是，鉴定出 319 个参与碳代谢的差异表达 CAZymes。通过 qRT-PCR 进一步验证了六个代表性 CAZymes 和漆酶基因的表达模式。酶活性结果表明，蓝光刺激下原基分化为子实体时 CAZymes 和漆酶的活性受到影响。综上所述，获得了蓝光刺激后杏鲍菇原基分化为子实体阶段的综合转录组和 CAZymes。从这个综合系统中获得的生物学见解为未来对这种商业上重要的蘑菇进行基因组研究提供了有价值的资源。
