Department of Medical Genetics, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Department of Medical Genetics, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran; Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
Pregnancy Hypertens. 2019 Apr;16:75-78. doi: 10.1016/j.preghy.2019.03.004. Epub 2019 Mar 11.
MicroRNAs (miRNAs, miRs) are small regulatory non-coding RNAs that regulate gene expression by incomplete complementary attachment to the 3'UTR, 5'UTR, ORF and promoter regions of target mRNAs. We compared plasma levels of miR-210-3p and miR-517c-3p as cell-free microRNAs (cfmiRNAs) in preeclamptic (n = 20) and healthy women (n = 20). These miRs are responsible for cell growth and proliferation, placental hypoxia, immune response and apoptosis. We found higher expression levels of miR-210 and miR-517c in preeclamptic cases (+3.34 and +2.27 fold change, respectively). This is the first study that evaluates the plasma levels of miR-517c in preeclamptic cases by real time PCR (RT-PCR) technique. This study can lead to new opportunities for research about the roles of miRNAs in preeclampsia etiology or new biomarkers.
微小 RNA（miRNA，miRs）是小的调节性非编码 RNA，通过不完全互补附着到靶 mRNA 的 3'UTR、5'UTR、ORF 和启动子区域来调节基因表达。我们比较了子痫前期（n=20）和健康女性（n=20）的血浆中 miR-210-3p 和 miR-517c-3p 作为细胞游离 miRNA（cfmiRNA）的水平。这些 miR 负责细胞生长和增殖、胎盘缺氧、免疫反应和细胞凋亡。我们发现子痫前期病例中 miR-210 和 miR-517c 的表达水平更高（分别增加了 3.34 倍和 2.27 倍）。这是首次通过实时 PCR（RT-PCR）技术评估子痫前期病例中 miR-517c 的血浆水平。这项研究可以为 miRNA 在子痫前期病因学或新生物标志物中的作用的研究提供新的机会。
