检测子痫前期患者循环 miR-517c-3p 和 miR-210-3p 的水平。
Quantification of circulating miR-517c-3p and miR-210-3p levels in preeclampsia.
机构信息
Department of Medical Genetics, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Department of Medical Genetics, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran; Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
出版信息
Pregnancy Hypertens. 2019 Apr;16:75-78. doi: 10.1016/j.preghy.2019.03.004. Epub 2019 Mar 11.
MicroRNAs (miRNAs, miRs) are small regulatory non-coding RNAs that regulate gene expression by incomplete complementary attachment to the 3'UTR, 5'UTR, ORF and promoter regions of target mRNAs. We compared plasma levels of miR-210-3p and miR-517c-3p as cell-free microRNAs (cfmiRNAs) in preeclamptic (n = 20) and healthy women (n = 20). These miRs are responsible for cell growth and proliferation, placental hypoxia, immune response and apoptosis. We found higher expression levels of miR-210 and miR-517c in preeclamptic cases (+3.34 and +2.27 fold change, respectively). This is the first study that evaluates the plasma levels of miR-517c in preeclamptic cases by real time PCR (RT-PCR) technique. This study can lead to new opportunities for research about the roles of miRNAs in preeclampsia etiology or new biomarkers.
微小 RNA（miRNA，miRs）是小的调节性非编码 RNA，通过不完全互补附着到靶 mRNA 的 3'UTR、5'UTR、ORF 和启动子区域来调节基因表达。我们比较了子痫前期（n=20）和健康女性（n=20）的血浆中 miR-210-3p 和 miR-517c-3p 作为细胞游离 miRNA（cfmiRNA）的水平。这些 miR 负责细胞生长和增殖、胎盘缺氧、免疫反应和细胞凋亡。我们发现子痫前期病例中 miR-210 和 miR-517c 的表达水平更高（分别增加了 3.34 倍和 2.27 倍）。这是首次通过实时 PCR（RT-PCR）技术评估子痫前期病例中 miR-517c 的血浆水平。这项研究可以为 miRNA 在子痫前期病因学或新生物标志物中的作用的研究提供新的机会。
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