Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Department of Biochemistry, Faculty of Pharmacy, Trakya University, Edirne, Turkey.
J Obstet Gynaecol. 2021 Aug;41(6):910-914. doi: 10.1080/01443615.2020.1820465. Epub 2020 Nov 24.
The present study aimed to identify the differential expression profiles of microRNAs in the plasma between patients with preeclampsia (PE) and healthy pregnancies using quantitative real-time PCR. The expression profiles of 32 miRNAs in maternal plasma from 31 patients with PE and 32 healthy pregnancies were evaluated. The expression levels of eight miRNAs including miR-210, miR-375, miR-197-3p, miR-132-3p, miR-29a-3p, miR-328, miR-24-3p, and miR-218-5p were significantly upregulated and the expression levels of three miRNAs, including miR-302b-3p, miR-191-5p, and miR-17-5p, were significantly downregulated in patients with preeclampsia when compared to healthy pregnant women. In conclusion, we identified 11 miRNAs that may be potential biomarkers for non-invasive diagnosis and a pivotal role in the prediction of PE. Considering the small cohort of patients, further studies with larger samples from different gestational stages are necessary to confirm our findings.IMPACT STATEMENT The alterations in the release pattern of placenta-specific miRNAs detected in maternal serum have been found to be associated with pregnancy-related complications such as preeclampsia (PE). In the present study, the release pattern of seven miRNAs had consistency and two of them had inconsistency with previous researches. Moreover, two novel miRNAs were also defined to demonstrate the interrelationship between PE and miRNAs. The identification of 11 miRNAs that may be potential biomarkers for non-invasive diagnosis and a pivotal role in the prediction of PE. Considering the small cohort of patients, further studies with larger samples from different gestational stages are necessary to confirm our findings.
本研究旨在利用定量实时 PCR 技术鉴定子痫前期（PE）患者血浆中 microRNA 的差异表达谱。评估了 31 例 PE 患者和 32 例健康妊娠孕妇血浆中 32 种 microRNA 的表达谱。结果显示，8 种 microRNA（包括 miR-210、miR-375、miR-197-3p、miR-132-3p、miR-29a-3p、miR-328、miR-24-3p 和 miR-218-5p）的表达水平明显上调，而 miR-302b-3p、miR-191-5p 和 miR-17-5p 的表达水平则明显下调。结论：我们鉴定出 11 种可能成为非侵入性诊断的潜在生物标志物，并在预测 PE 中具有重要作用。鉴于患者样本量较小，需要进一步进行不同妊娠阶段的更大样本研究来证实我们的发现。
在母体血清中检测到胎盘特异性 microRNA 的释放模式改变与子痫前期（PE）等与妊娠相关的并发症有关。在本研究中，七种 microRNA 的释放模式具有一致性，两种 microRNA 的释放模式与之前的研究不一致。此外，还确定了两种新的 microRNA，以展示 PE 与 microRNA 之间的相互关系。鉴定出 11 种可能成为非侵入性诊断的潜在生物标志物，并在预测 PE 中具有重要作用。鉴于患者样本量较小，需要进一步进行不同妊娠阶段的更大样本研究来证实我们的发现。
