Czech Academy of Sciences, Institute of Analytical Chemistry , Brno 61142 , Czech Republic.
CEITEC Masaryk University Brno 60177 , Czech Republic.
Anal Chem. 2019 Jun 4;91(11):7047-7053. doi: 10.1021/acs.analchem.8b05860. Epub 2019 May 14.
We have developed a new separation device to concentrate and collect ions from several milliliter sample volumes to microliter fractions. Unlike most conventional platforms, this device has circular architecture. The electrophoretic migration operates from the outer perimeter toward the center. Separations can be performed both in continuous (zone electrophoresis) and discontinuous (moving boundary) electrolyte systems. We use a discontinuous electrolyte system comprising a leading and a terminating electrolyte to concentrate samples containing small organic anions and DNA fragment. The agarose gel stabilizes the boundary between the leading and terminating electrolytes. The milliliter volume sample is mixed with the terminating electrolyte and migrates through the gel toward the center. The concentrated total sample is collected in microliter fraction at the center. The potential for preparative concentration of DNA is demonstrated using a DNA ladder. Because zone migration accelerates as it moves toward the center, we named this method Epitachophoresis from the Greek word "επιταχυνω (epitachýnο)", meaning "acceleration". To the best of our knowledge, this unique circular architecture has not been previously described.
我们开发了一种新的分离装置，可从几毫升的样品体积浓缩和收集离子到微升级分。与大多数传统平台不同，该装置具有圆形架构。电泳迁移从外周边向中心进行。可以在连续（区带电泳）和不连续（移动边界）电解质系统中进行分离。我们使用包含先导和终止电解质的不连续电解质系统来浓缩含有小有机阴离子和 DNA 片段的样品。琼脂糖凝胶稳定先导和终止电解质之间的边界。将毫升体积的样品与终止电解质混合，并通过凝胶向中心迁移。浓缩的总样品在中心以微升级分收集。使用 DNA 梯来证明 DNA 的制备浓缩的潜力。由于区带迁移在向中心移动时会加速，因此我们从希腊语单词“επιταχυνω（epitachýnο）”中命名这种方法为 Epitachophoresis，意思是“加速”。据我们所知，这种独特的圆形架构以前没有被描述过。
