Tarafdar Avijit, Vishwakarma Harinder, Gothandapani S, Bhati Meenal, Biswas Koushik, Prakash Arul, Chaturvedi Uttara, Solanke Amolkumar U, Padaria Jasdeep Chatrath
National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, 110012 India.
3 Biotech. 2019 May;9(5):180. doi: 10.1007/s13205-019-1708-6. Epub 2019 Apr 17.
mediated method was used to transform Indian elite wheat genotype HD2894 with herbicide-tolerant - (5-enolpyruvylshikimate-3-phosphate synthase) gene. The apical meristems of germinated seeds were targeted for introgression of transgene. The obtained T plants were screened by spraying 1% glyphosate and only positive transformants survived. The presence of transgene was also confirmed by PCR and Southern hybridization. Using this method, 3.07% transformation rate was observed. To identify transgenic lines carrying stably integrated - gene, the transgenic populations were screened in T generation using 1% glyphosate and lines with 100% survival were considered as homozygous. No significant morpho-physiological variations were observed within the transgenic lines as compared to non-transgenic plants. The present study resulted in herbicide-tolerant transgenic wheat and provides a valuable tool for development of wheat genetic transformation.
采用介导法将耐除草剂基因（5-烯醇丙酮酰莽草酸-3-磷酸合酶）导入印度优良小麦基因型HD2894中。以萌发种子的顶端分生组织作为转基因导入的靶点。通过喷洒1%的草甘膦对获得的T0植株进行筛选，只有阳性转化体存活。还通过PCR和Southern杂交证实了转基因的存在。采用这种方法，观察到转化率为3.07%。为了鉴定携带稳定整合基因的转基因株系，在T1代用1%的草甘膦对转基因群体进行筛选，存活率为100%的株系被视为纯合子。与非转基因植株相比，转基因株系内未观察到明显的形态生理变异。本研究获得了耐除草剂转基因小麦，为小麦遗传转化的发展提供了一个有价值的工具。
