Department of Otolaryngology‑Head and Neck Surgery, Nara Medical University, Kashihara, Nara 634‑8522, Japan.
Department of Biochemistry, Nara Medical University, Kashihara, Nara 634‑8522, Japan.
Oncol Rep. 2019 Jul;42(1):436-442. doi: 10.3892/or.2019.7137. Epub 2019 Apr 25.
Identifying the key molecules that enhance chemo‑ and radiosensitivity in head and neck squamous cell carcinoma (HNSCC) as well as reliable biomarkers for predicting recurrence and metastasis would be desirable to improve the prognosis of HNSCC. Previously, we have reported that Regenerating gene III (REG III) expression was associated with an improved survival rate for patients with HNSCC. In addition, resveratrol (3,4',5‑trihydroxystilbene) significantly increased REG III expression in HNSCC cells, and significantly inhibited cell growth, enhanced chemo‑ and radiosensitivity, and blocked the cancer invasion of HNSCC cells in vitro. In the present study, the effect of resveratrol on cancer progression in HNSCC was investigated in vivo using a xenograft nude mouse model. The results revealed that resveratrol increased the mRNA level of REG III in vivo, which was in agreement with our previous in vitro findings. Furthermore, REG III increased the antitumor effect of radiation or cisplatin in vivo, and resveratrol sensitized HNSCC to irradiation and cisplatin in vivo. These results indicated that resveratrol could increase the efficacy of cisplatin and irradiation through the REG III expression pathway, resulting in the inhibition of HNSCC progression in vivo.
确定能够增强头颈部鳞状细胞癌 (HNSCC) 化疗和放疗敏感性的关键分子，以及可靠的预测复发和转移的生物标志物，将有助于改善 HNSCC 的预后。此前，我们报道过，再生基因 III (REG III) 的表达与 HNSCC 患者的生存率提高有关。此外，白藜芦醇 (3,4',5-三羟基二苯乙烯) 可显著增加 HNSCC 细胞中 REG III 的表达，并显著抑制细胞生长，增强化疗和放疗敏感性，阻断 HNSCC 细胞的体外侵袭。在本研究中，使用异种移植裸鼠模型研究了白藜芦醇对 HNSCC 进展的体内作用。结果表明，白藜芦醇在体内增加了 REG III 的 mRNA 水平，这与我们之前的体外研究结果一致。此外，REG III 增加了体内辐射或顺铂的抗肿瘤作用，而白藜芦醇在体内使 HNSCC 对辐射和顺铂敏感。这些结果表明，白藜芦醇可以通过 REG III 表达途径增加顺铂和辐射的疗效，从而抑制体内 HNSCC 的进展。
