Unit of Botany and Plant Physiology, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, Al. 29 Listopada 54, 31-425, Kraków, Poland.
Unit of Botany and Plant Physiology, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, Al. 29 Listopada 54, 31-425, Kraków, Poland.
Ecotoxicol Environ Saf. 2019 Sep 30;180:12-22. doi: 10.1016/j.ecoenv.2019.04.059. Epub 2019 May 3.
The study aimed at comparing metabolic reactions of a halophyte Aster tripolium to abiotic stresses. Profiling of endogenous phytohormones, soluble carbohydrates and stress-related amino acids was conducted in plants exposed to moderate and high salinity (150 and 300 mM NaCl), and heavy metal salts CdCl or PbCl (100 and 200 μM). High NaCl and Pb doses inhibited growth of A. tripolium (Stress Tolerance Index STI) of 37% and 32-35%, respectively. The plants tolerated moderate salinity and Cd (STI = 91% and STI = 83-96%, respectively). Toxic metals accumulated mainly in the roots but Cd translocation to the shoots was also observed. The stressors did not affect total concentrations of the main growth promoting phytohormones but we observed enhanced deactivation of auxins and gibberellins, and reduced accumulation of jasmonate precursor. ABA content increased under stress except for moderate salinity. A common reaction was also activation of osmotic adjustment, however it was disparately manifested under salinity and metallic stress. The distinct responses to salinity and metallic stresses involved changes in carbohydrate profile and altered interplay between salicylic acid content and the pool of active gibberellins. The content of active jasmonates diversified A. tripolium reactions to salt excess and each of the heavy metals. This parameter was linked to the accumulation of ethylene precursor. The results of the study can be used to decipher potential co-tolerance mechanism of this halophyte species to multiple environmental stresses.
本研究旨在比较盐生植物蒲公英对非生物胁迫的代谢反应。在暴露于适度和高盐度（150 和 300 mM NaCl）以及重金属盐 CdCl 或 PbCl（100 和 200 μM）的植物中，对内源植物激素、可溶性碳水化合物和应激相关氨基酸进行了分析。高 NaCl 和 Pb 剂量分别抑制了 A. tripolium 的生长（胁迫耐受指数 STI）为 37%和 32-35%。植物耐受适度盐度和 Cd（STI = 91%和 STI = 83-96%）。有毒金属主要积累在根部，但也观察到 Cd 向地上部分的转运。胁迫因素不影响主要生长促进植物激素的总浓度，但我们观察到生长素和赤霉素的失活增强，茉莉酸前体的积累减少。除适度盐度外，胁迫下 ABA 含量增加。渗透调节的激活也是一种常见反应，但在盐胁迫和金属胁迫下表现不同。对盐胁迫和金属胁迫的不同反应涉及到碳水化合物谱的变化以及水杨酸含量和活性赤霉素库之间相互作用的改变。活性茉莉酸的含量使 A. tripolium 对盐过量和每种重金属的反应多样化。该参数与乙烯前体的积累有关。本研究的结果可用于破译该盐生植物对多种环境胁迫的潜在共耐受机制。
