Nakamura A, Uozumi T, Beppu T
Eur J Biochem. 1987 Apr 15;164(2):317-20. doi: 10.1111/j.1432-1033.1987.tb11060.x.
The nucleotide sequence of an endolytic cellulase gene of Bacillus subtilis was determined and compared with the NH2-terminal amino acid sequence of the purified enzyme. The mature protein appeared to be extended by a signal sequence of 36 amino acids. The putative AUG initiation codon was preceded by a sigma 43-type promoter of B. subtilis and an AAGGAGG sequence, typical of procaryotic ribosomal binding sites. Partial homology of amino acid sequences was found between B. subtilis cellulase and an alkalophilic Bacillus cellulase.
测定了枯草芽孢杆菌内切纤维素酶基因的核苷酸序列，并将其与纯化酶的氨基末端氨基酸序列进行了比较。成熟蛋白似乎由一个36个氨基酸的信号序列延伸。假定的AUG起始密码子之前是枯草芽孢杆菌的σ43型启动子和一个AAGGAGG序列，这是原核生物核糖体结合位点的典型序列。在枯草芽孢杆菌纤维素酶和嗜碱芽孢杆菌纤维素酶之间发现了氨基酸序列的部分同源性。
