Bownes M, Rembold H
Eur J Biochem. 1987 May 4;164(3):709-12. doi: 10.1111/j.1432-1033.1987.tb11184.x.
Using combined gas chromatography/selected-ion mass spectroscopy, the titer of juvenile hormone was determined for whole-body extracts at various morphologically defined stages of the life cycle of Drosophila melanogaster. Only juvenile hormone III (JH-III) was detected. JH-III is present in early metamorphosis but by mid-metamorphosis it is below the level of detection (0.05 pmol/g). It then increases as the pharate adult matures and rises dramatically, beginning just prior to eclosion and reaching 5-7 pmol/g shortly after eclosion. The titer then begins to fall again as the adults mature in both males and females, though the decrease is more rapid in females. Preliminary studies show that low levels of JH-III are present during all the larval instars but are absent from eggs.
使用气相色谱/选择离子质谱联用技术，测定了黑腹果蝇生命周期中各个形态学定义阶段的全身提取物中保幼激素的滴度。仅检测到保幼激素III（JH-III）。JH-III在变态初期存在，但到变态中期其含量低于检测水平（0.05 pmol/g）。然后随着蛹期成虫成熟，其含量增加，并在羽化前急剧上升，羽化后不久达到5 - 7 pmol/g。随着雌雄成虫成熟，该滴度随后又开始下降，不过雌性下降得更快。初步研究表明，在所有幼虫龄期都存在低水平的JH-III，但卵中没有。
