促黄体生成素释放激素（LHRH）诱导大鼠颗粒细胞释放花生四烯酸。钙和蛋白激酶C的作用。

Luteinizing hormone-releasing hormone (LHRH)-induced arachidonic acid release in rat granulosa cells. Role of calcium and protein kinase C.

作者信息

Minegishi T, Wang J, Leung P C

出版信息

FEBS Lett. 1987 Apr 6;214(1):139-42. doi: 10.1016/0014-5793(87)80029-7.

Abstract

In rat granulosa cells prelabeled with [3H]arachidonic acid, addition of LHRH, A23187 or 12-0-tetradecanoyl phorbol-13-acetate (TPA) enhanced the release of [3H]arachidonic acid into the culture medium. The effect of A23187 was significant as early as 5 min and the lowest effective dose was 5 X 10(-8)/M. On the other hand, TPA was effective only at dosages greater than 10(-6)M. These results suggest that the stimulatory effect of LHRH on arachidonic acid release is coupled more tightly to a Ca2+-dependent rather than a protein kinase C-mediated pathway.

摘要

在用[3H]花生四烯酸预标记的大鼠颗粒细胞中，添加促黄体生成素释放激素（LHRH）、A23187或12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）可增强[3H]花生四烯酸释放到培养基中的量。A23187的作用早在5分钟时就很显著，最低有效剂量为5×10（-8）/M。另一方面，TPA仅在剂量大于10（-6）M时才有效。这些结果表明，LHRH对花生四烯酸释放的刺激作用与钙依赖性途径的耦合比与蛋白激酶C介导的途径更紧密。