Ohki M, Uchida H, Tamura F, Ohki R, Nishimura S
J Bacteriol. 1987 May;169(5):1917-22. doi: 10.1128/jb.169.5.1917-1922.1987.
Temperature-sensitive dnaJ mutants of Escherichia coli showed a thermosensitive defect in the synthesis of beta-galactosidase. Synthesis of the lac mRNA was greatly reduced at the restrictive temperature. The mutants were also conditionally defective in the synthesis of a subset of membrane proteins such as succinate dehydrogenase, whereas the synthesis of anthranilate synthetase, encoded by trpED, as well as that of most cellular proteins, was unaffected at the restrictive temperature. The defect was specific for the dnaJ mutants among several dna mutants which are known to be involved in the initiation of DNA synthesis: dnaK, dnaA, and dnaB mutants synthesized each of these proteins normally even at the restrictive temperature. At the restrictive temperature, growth of the dnaJ mutants was arrested at a specific stage of the cell cycle.
大肠杆菌的温度敏感型dnaJ突变体在β-半乳糖苷酶合成中表现出温度敏感缺陷。在限制温度下，lac mRNA的合成大幅减少。这些突变体在琥珀酸脱氢酶等一部分膜蛋白的合成中也存在条件性缺陷，而由trpED编码的邻氨基苯甲酸合成酶以及大多数细胞蛋白的合成在限制温度下不受影响。在已知参与DNA合成起始的几个dna突变体中，该缺陷对dnaJ突变体具有特异性：dnaK、dnaA和dnaB突变体即使在限制温度下也能正常合成这些蛋白质中的每一种。在限制温度下，dnaJ突变体的生长在细胞周期的特定阶段被阻断。
