Georgopoulos C, Tilly K, Drahos D, Hendrix R
J Bacteriol. 1982 Mar;149(3):1175-7. doi: 10.1128/jb.149.3.1175-1177.1982.
B66.0 is one of the most abundant proteins of Escherichia coli. Its relative rate of synthesis is highly regulated depending on temperature and the growth rate of the culture. We identified the B66.0 protein to be the dnaK+ structural gene product since dnaK756 mutant bacteria synthesized a B66.0 protein with a more acidic isoelectric point.
B66.0是大肠杆菌中含量最丰富的蛋白质之一。其相对合成速率根据温度和培养物的生长速率受到高度调控。我们鉴定出B66.0蛋白是dnaK +结构基因产物,因为dnaK756突变细菌合成的B66.0蛋白具有更酸性的等电点。