从自制四川泡菜中分离得到的植物乳杆菌 HY 所产胞外多糖的纯化与特性研究。
Purification and characterization of an exopolysaccharide produced by Lactobacillus plantarum HY isolated from home-made Sichuan Pickle.
机构信息
College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048, China.
出版信息
Int J Biol Macromol. 2019 Aug 1;134:516-526. doi: 10.1016/j.ijbiomac.2019.05.010. Epub 2019 May 4.
In the present study, the exopolysaccharide (EPS) producing strain HY was isolated from home-made Sichuan Pickle and identified as Lactobacillus plantarum. The EPS was purified by ethanol precipitation, and fractionation by column chromatography, and the yield of purified HY EPS was 1.423 g/L. The molecular weight of the purified HY EPS was 9.549 × 10 Da and composed of mannose, galactose, glucuronic acid and glucose in a mass percentage of 72.99%, 17.27%, 6.99% and 2.75%, respectively. FT-IR spectrum exhibited the existence of carboxyl and hydroxyl group. Partial sugar residues and glycosidic bond present in HY EPS could be identified by NMR spectroscopy, i.e. α-d-glucose and α-d-mannose residues. The degradation temperature (320 °C) and melting point (122.78 °C) of the HY EPS were determined by thermogravimetric analysis (TGA) and differential scanning calorimeter analysis (DSC). A highly porous structure of EPS was observed by SEM, and the AFM analysis of the EPS revealed presence of spherical lumps. Meanwhile, the in vitro biological activity assays of HY EPS showed considerable antioxidant and α-amylase inhibitory activities, suggesting its promising potential to be applied in the food and pharmaceuticals industry.
在本研究中，从自制四川泡菜中分离出一株产胞外多糖（EPS）的菌株 HY，鉴定为植物乳杆菌。采用乙醇沉淀和柱层析对 EPS 进行分离纯化，得到的 HY EPS 产量为 1.423g/L。HY EPS 的分子量为 9.549×10 Da，由甘露糖、半乳糖、葡萄糖醛酸和葡萄糖组成，质量百分比分别为 72.99%、17.27%、6.99%和 2.75%。FT-IR 光谱显示存在羧基和羟基。通过 NMR 光谱鉴定了 HY EPS 中部分糖残基和糖苷键的存在，即α-d-葡萄糖和α-d-甘露糖残基。通过热重分析（TGA）和差示扫描量热法分析（DSC）确定 HY EPS 的降解温度（320°C）和熔点（122.78°C）。通过 SEM 观察到 EPS 的高度多孔结构，通过 AFM 分析发现 EPS 存在球形块状物。同时，HY EPS 的体外生物活性测定显示出相当的抗氧化和α-淀粉酶抑制活性，表明其在食品和制药工业中有应用的潜力。
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