Min Wei-Hong, Fang Xiao-Bin, Wu Tong, Fang Li, Liu Chun-Lei, Wang Ji
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China.
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China.
J Biosci Bioeng. 2019 Jun;127(6):758-766. doi: 10.1016/j.jbiosc.2018.12.004. Epub 2018 Dec 29.
An acidic exopolysaccharide (EPS) designated as EPS103 was isolated, purified and characterized from Lactobacillus plantarum JLAU103. EPS103 had a relatively lower molecular weight of 12.4 KDa and was consisted of arabinose, rhamnose, fucose, xylose, mannose, fructose, galactose, and glucose in an approximate molar ratio of 4.05: 6.04: 6.29: 5.22: 1.47: 5.21: 2.24: 1.83. A specific spectrogram of acidic polysaccharide was obtained by FT-IR analysis, and both α- and β-type configurations were presented in EPS103 based on the nuclear magnetic resonance spectroscopy. Microstructural analysis of EPS103 demonstrated a smooth and glittering cube structure, and presence of many homogeneous rod-shaped lumps. Comprehensive study of in vitro antioxidant activity indicated that EPS103 possess strong scavenging abilities against hydroxyl, ABTS, and DPPH radicals with the maximum of 80.4%, 65.5%, and 60.5%, respectively, at 10 mg/mL concentration. Furthermore, EPS103 also showed strong ferrous ions chelating activity and oxygen radical absorbance capacity. These results together indicated that the EPS103 isolated from L. plantarum JLAU103 has great potential for use as a natural antioxidant or functional additive in foods industry.
从植物乳杆菌JLAU103中分离、纯化并鉴定了一种名为EPS103的酸性胞外多糖（EPS）。EPS103的分子量相对较低，为12.4 kDa，由阿拉伯糖、鼠李糖、岩藻糖、木糖、甘露糖、果糖、半乳糖和葡萄糖组成，其摩尔比约为4.05:6.04:6.29:5.22:1.47:5.21:2.24:1.83。通过傅里叶变换红外光谱（FT-IR）分析获得了酸性多糖的特定光谱图，基于核磁共振光谱，EPS103中同时存在α型和β型构型。EPS103的微观结构分析显示为光滑闪亮的立方体结构，并存在许多均匀的棒状块状物。体外抗氧化活性的综合研究表明，EPS103对羟基、ABTS和DPPH自由基具有较强的清除能力，在10 mg/mL浓度下，清除率分别最高可达80.4%、65.5%和60.5%。此外，EPS103还表现出较强的亚铁离子螯合活性和氧自由基吸收能力。这些结果共同表明，从植物乳杆菌JLAU103中分离得到的EPS103在食品工业中作为天然抗氧化剂或功能性添加剂具有巨大的应用潜力。
