Martínez-Gutiérrez Emir, Cuervo-López Flor de María, Texier Anne-Claire, Gómez Jorge
Department of Biotechnology, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, Vicentina, Iztapalapa, C.P. 09340 Mexico City, Mexico.
3 Biotech. 2019 May;9(5):190. doi: 10.1007/s13205-019-1723-7. Epub 2019 Apr 26.
The aim of this study was to evaluate the physiologic behavior of sludge in the absence and presence of 2-chlorophenol (2-CP) with different electron donors (phenol, glucose, and acetate) during denitrification process. In batch assays with phenol in the presence of 2-CP, a significant decrease of phenol consumption efficiencies ( ) up to 99% was observed regarding the cultures without 2-CP. However, in most of the cases, nitrate consumption efficiencies ( ), and yields of nitrogen gas ( ) and bicarbonate ( ) were high, showing that the denitrifying respiratory process successfully occurred with phenol and 2-CP. The specific consumption rates of nitrate ( ) and phenol ( ) decreased up to 6.0 and 32.3 times, respectively. In assays with glucose in the presence of 2-CP, the denitrifying performance was not significantly altered in terms of efficiencies and product yields; however, was up to 1.6 times smaller than that obtained without 2-CP whereas was increased up to 1.17 times. In assays with acetate plus 2-CP, the , , and values remained high but 2-CP caused a decrease in . Moreover, and increased up to 1.4 and 2.0 times, respectively. These results show that the negative or positive effects of 2-CP on denitrification process depend on the type and concentration of electron source. The obtained physiologic and kinetic information might be useful to define strategies to maintain successful denitrification processes in wastewater treatment bioreactors fed with 2-CP.
本研究的目的是评估在反硝化过程中，在不存在和存在2-氯苯酚（2-CP）且有不同电子供体（苯酚、葡萄糖和乙酸盐）的情况下污泥的生理行为。在含有2-CP的苯酚分批试验中，与不含2-CP的培养物相比，观察到苯酚消耗效率显著降低（高达99%）。然而，在大多数情况下，硝酸盐消耗效率、氮气产量和碳酸氢盐产量都很高，表明苯酚和2-CP成功地发生了反硝化呼吸过程。硝酸盐和苯酚的比消耗速率分别降低了6.0倍和32.3倍。在含有2-CP的葡萄糖试验中，反硝化性能在效率和产物产量方面没有显著改变；然而，与不含2-CP时相比降低了1.6倍，而增加了1.17倍。在乙酸盐加2-CP的试验中，、和值仍然很高，但2-CP导致降低。此外，和分别增加了1.4倍和2.0倍。这些结果表明，2-CP对反硝化过程的负面或正面影响取决于电子源的类型和浓度。所获得的生理和动力学信息可能有助于确定在以2-CP为进料的废水处理生物反应器中维持成功反硝化过程的策略。
