Beckman Amy K, Ferrieri Patricia
Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis.
Lab Med. 2019 Oct 10;50(4):390-395. doi: 10.1093/labmed/lmz022.
The Verigene Enteric Pathogens Test (Luminex Corporation) is a polymerase chain reaction (PCR)/nucleic acid microarray-based assay targeting 8 bacterial and viral pathogens that cause diarrhea.
To compare traditional enteric culture methods with stool testing by Verigene EP (PCR/microarray).
Tests were performed using PCR/microarray between February and August 2016. All specimens also underwent culture for Salmonella and Shigella; specimens that tested positive for bacterial pathogen(s) had confirmatory cultures.
Valid results were obtained for 99.3% of the 3795 stool specimens. Among these, 497 (13.2%) specimens tested positive for at least 1 pathogen by PCR/microarray; 45.5% of these tested positive for 1 or more bacterial pathogens. Agreement between positive bacterial PCR/microarray results and culture-based testing was 85.3%. Compared with cultures, PCR/microarray demonstrated 95.2% and 87.5% sensitivity and 99.8% and 99.8% specificity for Salmonella and Shigella, respectively.
The Verigene EP generated evaluable results for most stool specimens tested and demonstrated good agreement with bacterial cultures.
Verigene肠道病原体检测（Luminex公司）是一种基于聚合酶链反应（PCR）/核酸微阵列的检测方法，可检测8种引起腹泻的细菌和病毒病原体。
比较传统肠道培养方法与Verigene EP（PCR/微阵列）粪便检测方法。
在2016年2月至8月期间使用PCR/微阵列进行检测。所有标本均进行沙门氏菌和志贺氏菌培养；检测出细菌病原体呈阳性的标本进行确证培养。
3795份粪便标本中有99.3%获得了有效结果。其中，497份（13.2%）标本通过PCR/微阵列检测至少1种病原体呈阳性；其中45.5%检测出1种或更多种细菌病原体呈阳性。细菌PCR/微阵列阳性结果与基于培养的检测结果之间的一致性为85.3%。与培养方法相比，PCR/微阵列对沙门氏菌和志贺氏菌的敏感性分别为95.2%和87.5%，特异性分别为99.8%和99.8%。
Verigene EP对大多数检测的粪便标本产生了可评估的结果，并与细菌培养结果具有良好的一致性。
