Herschlag Rachel, Escalante Cesar, de Souto Eliezer Rodrigues, Khankhum Surasak, Okada Ryo, Valverde Rodrigo A
Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA, 70803, USA.
Department of Agronomy, Universidade Estadual de Mariga, Parana, Brazil.
Arch Virol. 2019 Jul;164(7):1863-1868. doi: 10.1007/s00705-019-04270-5. Epub 2019 May 7.
Extraction and electrophoretic analysis of viral dsRNA from plants has been used successfully to detect infections by RNA viruses. We used this approach as an initial tool to test non-cultivated plant species for the presence of endornaviruses. Foliar samples were collected from symptomless plants in various locations within East Baton Rouge Parish, Louisiana, USA, and tested for viral dsRNA. After testing 208 plant species belonging to 74 families, five (Geranium carolinianum, Hydrocotyle umbellata, H. prolifera, Sorghum halepense, and Sisyrinchium atlanticum) yielded dsRNAs similar in size to the dsRNAs of members of the family Endornaviridae. The endornavirus nature of the dsRNAs was confirmed by reverse-transcription PCR (RT-PCR) and sequencing the RT-PCR products. Sequence data were used to determine relationships of the putative endornaviruses to members of the family Endornaviridae. The putative endornaviruses were detected in both native and introduced plants species. This is the first survey on the occurrence of endornaviruses in non-cultivated plant species.
从植物中提取病毒双链RNA并进行电泳分析已成功用于检测RNA病毒感染。我们采用这种方法作为初步工具，检测未栽培植物物种中是否存在内源病毒。从美国路易斯安那州东巴吞鲁日教区不同地点的无症状植物上采集叶片样本，并检测病毒双链RNA。在检测了属于74个科的208种植物后，有5种植物（卡罗莱纳老鹳草、伞形天胡荽、多育天胡荽、黑高粱和大西洋蓝眼草）产生了大小与内病毒科成员双链RNA相似的双链RNA。通过逆转录PCR（RT-PCR）和对RT-PCR产物进行测序，证实了双链RNA的内源病毒性质。序列数据用于确定推定的内源病毒与内病毒科成员之间的关系。在本地和外来植物物种中均检测到了推定的内源病毒。这是首次对未栽培植物物种中内源病毒的发生情况进行调查。
