Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA, USA.
Laboratory of Molecular and Cellular Biology, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, 183-8509, Japan.
Virus Res. 2020 Oct 15;288:198116. doi: 10.1016/j.virusres.2020.198116. Epub 2020 Aug 11.
A putative endornavirus was detected in Carolina geranium (Geranium carolinianum) in Louisiana, USA. The virus was provisionally named Geranium carolinianum endornavirus 1 (GcEV1). The viral RNA was sequenced, and it consisted of 14,625 nt containing a single ORF coding a putative polyprotein of 4815 aa with conserved domains for a helicase 1, peptidase C97, glycosyl transferase GTB-type, and RNA-dependent RNA polymerase 2. The 5'end consisted of 130 nt while the 3'end consisted of 54 nt ending in nine cytosine residues. The closest relative to GcEV1 was Phaseolus vulgaris endornavirus 3. In phylogenetic analyses, GcEV1 clustered with members of the genus Alphaendornavirus. GcEV1 was detected in 57 of 60 G. carolinianum plants collected from three distinct agroecosystems. The virus was not detected in eight other species of the genus Geranium. There was no association of a particular phenotypic trait of the host with the presence or absence of the virus. GcEV1 was transmitted at a rate of 100% in seeds of a self-pollinated G. carolinianum plant.
在美国路易斯安那州的卡罗莱纳天竺葵（Geranium carolinianum）中检测到一种假定的内源性病毒。该病毒被临时命名为卡罗莱纳天竺葵内源性病毒 1（GcEV1）。病毒的 RNA 进行了测序，它由 14625 个核苷酸组成，包含一个单一的 ORF，编码一个推定的 4815 个氨基酸的多蛋白，具有保守的结构域，包括解旋酶 1、肽酶 C97、糖基转移酶 GTB 型和 RNA 依赖性 RNA 聚合酶 2。5'端由 130 个核苷酸组成，3'端由 54 个核苷酸组成，以九个胞嘧啶残基结尾。与 GcEV1 最接近的是菜豆内源性病毒 3。在系统发育分析中，GcEV1 与α内源性病毒属的成员聚类在一起。从三个不同的农业生态系统中采集的 60 株卡罗莱纳天竺葵植物中有 57 株检测到 GcEV1。在其他 8 种天竺葵属植物中未检测到该病毒。宿主的特定表型特征与病毒的存在与否之间没有关联。在自花授粉的卡罗莱纳天竺葵植物的种子中，GcEV1 的传播率为 100%。
