Laboratory of Parasitology and Parasitic Diseases, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Faculty of Veterinary Medicine, University of Teramo, Località Piano D'Accio snc., Teramo, Italy.
PLoS Negl Trop Dis. 2019 May 8;13(5):e0007003. doi: 10.1371/journal.pntd.0007003. eCollection 2019 May.
The present study investigated the presence of endo- and ecto-parasites, and vector-borne pathogens, in dogs from four islands of Greece. A total of 200 (123 owned and 77 sheltered) dogs were examined with different microscopic, serological and molecular methods. Of the examined dogs, 130 (65%) were positive for one or more parasites and/or vector-borne pathogens. The most common zoonotic intestinal helminths recorded were Ancylostomatidae (12.5%) and Toxocara canis (3.5%). Ninety-three dogs (46.5%) seroreacted to Rickettsia conorii. Twenty-two (11%) of them were also PCR positive and 7 (3.5%) showed corpuscles suggestive of Rickettsia spp. on the blood smears. Nineteen dogs (9.5%) were seropositive for Ehrlichia canis, three of them being also PCR positive. Dogs positive for Anaplasma phagocytophilum-Anaplasma platys (1%), Dirofilaria immitis (0.5%) and Babesia canis (0.5%) were also found. Fleas and ticks were recorded in 53 (26.5%) and 50 (25%) dogs, respectively, and all specimens were identified as Ctenocephalides felis felis and Rhipicephalus sanguineus sensu lato. Binary multiple univariate Generalized Linear Models were used to investigate factors and clinical signs related to the recorded positivity, while the association of specific signs with the pathogens was evaluated using tests of independence. Knowledge of occurrence and impact of zoonotic parasites and vector-borne pathogens in dog populations is crucial to prevent the infection in animals and people, and to control the risk of spreading of these pathogens in endemic and non-endemic areas.
本研究调查了希腊四个岛屿的犬只体内的内寄生虫和外寄生虫以及媒介传播病原体的存在情况。使用不同的显微镜、血清学和分子方法检查了总共 200 只（123 只家养犬和 77 只收容犬）。在所检查的犬中，有 130 只（65%）至少有一种或多种寄生虫和/或媒介传播病原体呈阳性。记录到的最常见的人兽共患肠道蠕虫是Ancylostomatidae（12.5%）和 Toxocara canis（3.5%）。93 只犬（46.5%）对 Rickettsia conorii 血清学呈阳性反应。其中 22 只（11%）也通过 PCR 呈阳性，7 只（3.5%）血涂片上出现提示 Rickettssia 属的细胞。19 只犬（9.5%）对 Ehrlichia canis 呈血清阳性，其中 3 只也通过 PCR 呈阳性。还发现了犬埃立克体-无形体（1%）、犬心丝虫（0.5%）和犬巴贝斯虫（0.5%）阳性犬。在 53 只（26.5%）和 50 只（25%）犬中分别记录到跳蚤和蜱虫，所有标本均鉴定为猫栉首蚤和血红扇头蜱。使用二元多变量广义线性模型来研究与记录的阳性结果相关的因素和临床症状，而使用独立性检验来评估特定症状与病原体的关联。了解犬群中 zoonotic 寄生虫和媒介传播病原体的发生和影响对于预防动物和人类感染、控制这些病原体在流行和非流行地区的传播风险至关重要。
