Gravekamp C, van den Bulck L P, Vijg J, van de Griend R J, Bolhuis R L
Nat Immun Cell Growth Regul. 1987;6(1):28-36.
The levels of c-myc mRNA and interleukin-2 receptors (IL-2 Rec) were studied in human peripheral blood lymphocytes (PBL); mature CD2+,CD3+ T cell clones and CD2+,CD3- natural killer (NK) cell clones, and CD2+,CD3+ and CD2-,CD3- T lymphoma cell lines. A transient induction of the expression of c-myc and IL-2 Rec was observed in PBL after activation with phytohemagglutinin (PHA). Expression of c-myc and IL-2 Rec was also found in the CD2+,CD3+ and CD2+,CD3- clones. The CD2+,CD3+ showed higher levels of c-myc mRNA and IL-2 Rec than the CD2+,CD3- clones. In three T lymphoma cell lines constitutively high levels of c-myc mRNA but no IL-2 Rec were found. Only in JURKAT (CD2+,CD3+), c-myc mRNA levels could be further enhanced by PHA. These results suggest that in the presence of PHA, expression of c-myc and IL-2 Rec is induced via the CD3 receptor, and in the absence of PHA and/or the CD3 receptor alternative routes of induction are involved.
研究了人外周血淋巴细胞（PBL）、成熟的CD2⁺、CD3⁺ T细胞克隆和CD2⁺、CD3⁻自然杀伤（NK）细胞克隆以及CD2⁺、CD3⁺和CD2⁻、CD3⁻ T淋巴瘤细胞系中c-myc mRNA和白细胞介素-2受体（IL-2 Rec）的水平。在用植物血凝素（PHA）激活后，在PBL中观察到c-myc和IL-2 Rec表达的短暂诱导。在CD2⁺、CD3⁺和CD2⁺、CD3⁻克隆中也发现了c-myc和IL-2 Rec的表达。CD2⁺、CD3⁺克隆显示出比CD2⁺、CD3⁻克隆更高水平的c-myc mRNA和IL-2 Rec。在三种T淋巴瘤细胞系中，发现c-myc mRNA持续高水平表达，但未发现IL-2 Rec。仅在JURKAT（CD2⁺、CD3⁺）中，PHA可进一步提高c-myc mRNA水平。这些结果表明，在PHA存在的情况下，c-myc和IL-2 Rec的表达通过CD3受体诱导，而在没有PHA和/或CD3受体的情况下，涉及其他诱导途径。
