Malter J S, Reed J C, Kamoun M
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104.
J Immunol. 1988 May 1;140(9):3233-6.
Herein we studied the accumulation and decay of CD2 mRNA in human PBMC stimulated with PHA. The data show that CD2-specific messages are present in low quantities in resting PBMC and are rapidly increased by five- to sevenfold within 24 h of addition of optimal amounts of PHA. Similar induction of CD2 mRNA was seen after stimulation of PBMC with anti-CD3 mAb and PMA. Peak levels of CD2 message were maintained until 72 h post-stimulation and declined gradually thereafter. Despite stimulation with Staphylococcus aureus and PMA, purified B cells failed to demonstrate any CD2 mRNA. Unlike transferrin or IL-2R mRNA, Il-2 had no effect on the accumulation of CD2 messages in resting or PHA-stimulated PBMC. The time course of CD2 mRNA accumulation preceded lymphocyte proliferation and the appearance of additional cell surface CD2 Ag. The decay of CD2 mRNA was very rapid, with a t 1/2 of approximately 45 min. The protein synthesis inhibitor, cycloheximide, increased its half-time by fourfold to 3.5 h. The data imply the existence of a labile factor, dependent on protein synthesis that is important in the regulation of CD2 mRNA. Compared to other PHA-inducible lymphocyte genes, the kinetics of CD2 transcript accumulation are most reminiscent of the oncogenes N-ras and c-abl.
在此，我们研究了用PHA刺激的人外周血单核细胞（PBMC）中CD2 mRNA的积累和衰减。数据显示，静止的PBMC中存在少量的CD2特异性信息，在加入最佳量的PHA后24小时内迅速增加5至7倍。用抗CD3单克隆抗体和PMA刺激PBMC后，也观察到类似的CD2 mRNA诱导。CD2信息的峰值水平一直维持到刺激后72小时，此后逐渐下降。尽管用金黄色葡萄球菌和PMA刺激，纯化的B细胞仍未显示出任何CD2 mRNA。与转铁蛋白或IL-2R mRNA不同，IL-2对静止或PHA刺激的PBMC中CD2信息的积累没有影响。CD2 mRNA积累的时间进程先于淋巴细胞增殖和其他细胞表面CD2抗原的出现。CD2 mRNA的衰减非常迅速，半衰期约为45分钟。蛋白质合成抑制剂环己酰亚胺将其半衰期延长了四倍，至3.5小时。数据表明存在一种不稳定因子，它依赖于蛋白质合成，在CD2 mRNA的调节中起重要作用。与其他PHA诱导的淋巴细胞基因相比，CD2转录本积累的动力学最类似于癌基因N-ras和c-abl。
