Department of Psychiatry and Psychotherapy, Friedrich-Alexander-University Erlangen-Nuremberg, University Hospital Erlangen, Erlangen, Germany.
Department of Internal Medicine 3 - Rheumatology and Immunology, Friedrich-Alexander-University Erlangen-Nürnberg, University Hospital Erlangen, Erlangen, Germany.
Sci Rep. 2019 May 8;9(1):7089. doi: 10.1038/s41598-019-43607-7.
Microvesicles are small membranous particles generated during cellular activation or stress. The analysis of the content and the surface of microvesicles allow conclusions about the cells they are originating from and the underlying pathology. Therefore, CSF microvesicles have been suggested to be promising targets to monitor the (etio)pathology of neurodegenerative diseases. Microvesicles in the CSF of 15 patients with Alzheimer's disease and 15 controls were analyzed by flow cytometry regarding the levels of CD3, CD4, CD45, CD64, BACE1, Aβ, APP and tau. The results were replicated in a second cohort comprising 14 patients with Alzheimer's disease and 9 controls. The levels of tau and APP were reduced in microvesicles of Alzheimer's disease patients. A significant change was neither observed in the number of microvesicles nor in the expression of the other antigens. Tau and APP in microvesicles separated patients with Alzheimer's disease from controls with an AUC of 0.84 and 0.89 respectively. We conclude that tau and APP in CSF microvesicles are promising biomarkers which could directly provide information about the Alzheimer pathology on a cellular level.
微泡是细胞激活或应激时产生的小膜性颗粒。对微泡的内容物和表面进行分析，可以得出关于其来源细胞和潜在病理学的结论。因此，CSF 微泡被认为是监测神经退行性疾病（病因）病理学的有前途的靶点。通过流式细胞术分析了 15 例阿尔茨海默病患者和 15 名对照者 CSF 中的微泡，检测 CD3、CD4、CD45、CD64、BACE1、Aβ、APP 和 tau 的水平。结果在第二个包含 14 例阿尔茨海默病患者和 9 名对照者的队列中得到了复制。与对照组相比，阿尔茨海默病患者微泡中的 tau 和 APP 水平降低。微泡数量或其他抗原的表达均未观察到显著变化。tau 和 APP 在微泡中的分离使阿尔茨海默病患者与对照组区分开来，AUC 分别为 0.84 和 0.89。我们得出结论，CSF 微泡中的 tau 和 APP 是有前途的生物标志物，它们可以直接提供关于细胞水平阿尔茨海默病病理学的信息。
