Nellist Charlotte F, Vickerstaff Robert J, Sobczyk Maria K, Marina-Montes César, Wilson Fiona M, Simpson David W, Whitehouse Adam B, Harrison Richard J
Department of Genetics, Genomics and Breeding, NIAB EMR, New Road, East Malling, ME19 6BJ UK.
Hortic Res. 2019 May 1;6:60. doi: 10.1038/s41438-019-0136-4. eCollection 2019.
The cultivated strawberry, ( spp.) is the most economically important global soft fruit. , a water-borne oomycete causes economic losses in strawberry production globally. A bi-parental cross of octoploid cultivated strawberry segregating for resistance to . , the causative agent of crown rot disease, was screened using artificial inoculation. Multiple putative resistance quantitative trait loci (QTL) were identified and mapped. Three major effect QTL (, and ) explained 37% of the variation observed. There were no epistatic interactions detected between the three major QTLs. Testing a subset of the mapping population progeny against a range of isolates revealed no significant interaction ( = 0.0593). However, some lines showed higher susceptibility than predicted, indicating that additional undetected factors may affect the expression of some quantitative resistance loci. Using historic crown rot disease score data from strawberry accessions, a preliminary genome-wide association study (GWAS) of 114 individuals revealed an additional locus associated with resistance to . . Mining of the Hawaii 4 v1.1 genome revealed candidate resistance genes in the QTL regions.
栽培草莓（ spp.）是全球经济上最重要的软性水果。 ，一种水传卵菌在全球草莓生产中造成经济损失。利用人工接种对八倍体栽培草莓的一个对 具有抗性分离的双亲杂交组合进行筛选， 是冠腐病的病原体。鉴定并定位了多个假定的抗性数量性状位点（QTL）。三个主要效应QTL（ 、 和 ）解释了观察到的37%的变异。在这三个主要QTL之间未检测到上位性相互作用。用一系列 分离株对定位群体子代的一个子集进行测试，未发现显著相互作用（ = 0.0593）。然而，一些品系表现出比预期更高的易感性，这表明其他未检测到的因素可能影响一些数量抗性位点的表达。利用草莓种质的历史冠腐病评分数据，对114个个体进行的初步全基因组关联研究（GWAS）揭示了另一个与对 抗性相关的位点。对夏威夷4 v1.1基因组的挖掘揭示了QTL区域中的候选抗性基因。
