Department of Plant Sciences, Faculty of Biosciences (BIOVIT), Norwegian University of Life Sciences (NMBU), 1433 Ås, Norway.
Division of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research (NIBIO), 1433 Ås, Norway.
Int J Mol Sci. 2023 Jun 29;24(13):10851. doi: 10.3390/ijms241310851.
Crown rot, caused by , is a devastating disease of strawberry. While most commercial octoploid strawberry cultivars ( × Duch) are generally susceptible, the diploid species is a potential source of resistance genes to . We previously reported several genotypes with varying degrees of resistance to . To gain insights into the strawberry defence mechanisms, comparative transcriptome profiles of two resistant genotypes (NCGR1603 and Bukammen) and a susceptible genotype (NCGR1218) of were analysed by RNA-Seq after wounding and subsequent inoculation with Differential gene expression analysis identified several defence-related genes that are highly expressed in the resistant genotypes relative to the susceptible genotype in response to after wounding. These included putative disease resistance () genes encoding receptor-like proteins, receptor-like kinases, nucleotide-binding sites, leucine-rich repeat proteins, RPW8-type disease resistance proteins, and 'pathogenesis-related protein 1'. Seven of these -genes were expressed only in the resistant genotypes and not in the susceptible genotype, and these appeared to be present only in the genomes of the resistant genotypes, as confirmed by PCR analysis. We previously reported a single major gene locus () in that contributed resistance to . Here, we report that 4-5% of the genes (35-38 of ca 800 genes) in the locus are differentially expressed in the resistant genotypes compared to the susceptible genotype after inoculation with . In particular, we identified three defence-related genes encoding wall-associated receptor-like kinase 3, receptor-like protein 12, and non-specific lipid-transfer protein 1-like that were highly expressed in the resistant genotypes compared to the susceptible one. The present study reports several novel candidate disease resistance genes that warrant further investigation for their role in plant defence against .
根腐病，由 引起，是草莓的一种毁灭性疾病。虽然大多数商业八倍体草莓品种（ × Duch）普遍易感，但二倍体种 是 抗性基因的潜在来源。我们之前报道了几个对 具有不同程度抗性的 基因型。为了深入了解草莓的防御机制，我们通过 RNA-Seq 对两个抗性基因型（NCGR1603 和 Bukammen）和一个易感基因型（NCGR1218）进行了比较转录组分析，结果表明：在受伤后用 接种，与易感基因型相比，几个防御相关基因在抗性基因型中高度表达。这些基因包括编码受体样蛋白、受体样激酶、核苷酸结合位点、富含亮氨酸重复蛋白、RPW8 型抗病蛋白和“病程相关蛋白 1”的假定抗病（）基因。其中 7 个 -基因仅在抗性基因型中表达，而在易感基因型中不表达，而且这些基因似乎仅存在于抗性基因型的基因组中，这通过 PCR 分析得到了证实。我们之前报道了一个单一的主要基因座 （）在 中对 具有抗性。在这里，我们报告说，在接种 后，与易感基因型相比，抗性基因型中有 4-5%的基因（800 个基因中的 35-38 个）差异表达。特别是，我们鉴定了三个与防御相关的基因，它们分别编码细胞壁相关受体样激酶 3、受体样蛋白 12 和非特异性脂质转移蛋白 1 样，在抗性基因型中的表达水平明显高于易感基因型。本研究报道了几个新的候选抗病基因，它们值得进一步研究，以了解它们在植物防御中的作用。
