Caetano Liliana Aranha, Faria Tiago, Springer Jan, Loeffler Juergen, Viegas Carla
H&TRC- Health & Technology Research Center, ESTeSL- Escola Superior de Tecnologia da Saúde, Instituto Politécnico de Lisboa, Lisbon, Portugal.
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal.
Mycology. 2018 Nov 26;10(2):75-83. doi: 10.1080/21501203.2018.1551251. eCollection 2019 Jun.
This paper sought to address the prevalence of Mucorales in different indoor environments in Portugal. Environmental samples (183 in total) were collected at dwellings ( = 79) and workplaces (bakeries, swine farms, taxis, waste-sorting plants) ( = 93) by passive sampling using electrostatic dust collector (EDC), air-conditioning filters, litter, and/or raw materials. Samples were inoculated onto non-selective MEA and DG18 media and were screened for antifungal drug-resistance in azole-supplemented agar Sabouraud media. A probe-based Mucorales-specific real-time PCR assay (Muc18S) was used to detect Mucorales in complement to conventional culture-based methods. Mucorales order was found as more prevalent in air-conditioning filters from waste-sorting fork lifters (35.7%). Amongst Mucorales isolates able to grow in azole-supplemented media, 16 isolates of sp., sp. or sp. were not susceptible to 1 mg/L voriconazole, and four isolates of sp. or sp. were not susceptible to 4 mg/L itraconazole. In conclusion, combination of the culture-based and molecular methods proved to be reliable for Mucorales order identification in complex environmental samples.
本文旨在研究葡萄牙不同室内环境中毛霉目真菌的流行情况。通过使用静电集尘器（EDC）、空调过滤器、垃圾和/或原材料进行被动采样，在住宅（n = 79）和工作场所（面包店、养猪场、出租车、垃圾分类厂）（n = 93）共采集了183份环境样本。将样本接种到非选择性麦芽提取物琼脂（MEA）和DG18培养基上，并在添加了唑类的沙氏琼脂培养基上筛选抗真菌药物耐药性。基于探针的毛霉目特异性实时聚合酶链反应检测法（Muc18S）用于补充传统的基于培养的方法来检测毛霉目真菌。在垃圾分类叉车的空调过滤器中发现毛霉目真菌最为普遍（35.7%）。在能够在添加唑类的培养基中生长的毛霉目分离株中，16株根霉属、毛霉属或犁头霉属菌株对1 mg/L伏立康唑不敏感，4株根霉属或毛霉属菌株对4 mg/L伊曲康唑不敏感。总之，基于培养的方法和分子方法相结合被证明对于复杂环境样本中毛霉目真菌的鉴定是可靠的。
