Viegas Carla, Gomes Bianca, Dias Marta, Carolino Elisabete, Aranha Caetano Liliana
H&TRC-Health & Technology Research Center, ESTeSL-Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa, 1990-096 Lisbon, Portugal.
Public Health Research Centre, NOVA National School of Public Health, Universidade NOVA de Lisboa, 1099-085 Lisbon, Portugal.
Microorganisms. 2021 Oct 7;9(10):2112. doi: 10.3390/microorganisms9102112.
section is one of the sections more frequently related to respiratory symptoms and by other health outcomes. This study aimed to characterize section distribution in eleven firefighter headquarters (FFHs) to obtain an accurate occupational exposure assessment.
A sampling approach protocol was performed using active (impaction method) and passive sampling methods (floor surfaces swabs, electrostatic dust collectors (EDCs), and settled dust). All samples were analysed by culture-based methods and passive sampling was used for molecular detection of section Results: Of all the matrices, the highest counts of sp. were obtained on settled dust filters (3.37% malt extract agar-MEA, 19.09% dichloran glycerol-DG18) followed by cleaning cloths (1.67% MEA; 7.07% DG18). Among the genus, the section was predominant in Millipore and EDC samples in MEA (79.77% and 28.57%, respectively), and in swabs and settled dust filters in DG18 (44.76% and 30%, respectively). The section was detected more frequently in DG18 (33.01%) compared to MEA (0.33%). The section was observed in azole supplemented media (itraconazole and voriconazole) in several passive sampling methods employed and detected by qPCR in almost all passive samples, with EDCs being the matrix with the highest prevalence ( = 61; 67.8%).
This study confirms that sp. is widespread and the section is present in all FFHs. The presence of fungi potentially resistant to azoles in the FFHs was also observed. Further studies are needed to identify the best corrective and preventive measures to avoid this section contamination in this specific occupational environment.
该菌属是与呼吸道症状及其他健康结果关联更为频繁的菌属之一。本研究旨在描述11个消防员总部（FFH）中该菌属的分布情况，以获得准确的职业暴露评估。
采用主动（撞击法）和被动采样方法（地面擦拭样本、静电集尘器（EDC）和沉降灰尘）执行采样方案。所有样本均通过基于培养的方法进行分析，被动采样用于该菌属的分子检测。结果：在所有基质中，沉降灰尘过滤器上的该菌属计数最高（麦芽提取物琼脂 - MEA上为3.37%，二氯苯胺甘油 - DG18上为19.09%），其次是清洁布（MEA上为1.67%；DG18上为7.07%）。在该菌属中，该菌属在MEA的密理博和EDC样本中占主导（分别为79.77%和28.57%），在DG18的拭子和沉降灰尘过滤器中占主导（分别为44.76%和30%）。与MEA（0.33%）相比，该菌属在DG18中检测更为频繁（33.01%）。在采用的几种被动采样方法中，在添加唑类（伊曲康唑和伏立康唑）的培养基中观察到该菌属，并在几乎所有被动样本中通过qPCR检测到，其中EDC是患病率最高的基质（n = 61；67.8%）。
本研究证实该菌属广泛存在，且该菌属存在于所有消防员总部。还观察到消防员总部中存在对唑类有潜在抗性的真菌。需要进一步研究以确定最佳的纠正和预防措施，以避免在这种特定职业环境中该菌属的污染。
