一种用于诊断牛巴贝斯虫感染的酶联免疫吸附测定法。
An enzyme linked immunosorbent assay to diagnose Babesia bovis infection in cattle.
作者信息
Waltisbuhl D J, Goodger B V, Wright I G, Commins M A, Mahoney D F
出版信息
Parasitol Res. 1987;73(2):126-31. doi: 10.1007/BF00536468.
PMID:3106957
Abstract
The optimum gel filtration fraction from lysate of Babesia bovis infected erythrocytes was determined for use as an antigen in an ELISA to diagnose B. bovis infection in cattle. Of four enzyme labels tested, horseradish peroxidase was the most suitable. The assay is both sensitive and specific in detecting antibody for 2-4 years after a single infection. False positive reactions were obtained only with some sera from some Anaplasma marginale infected cattle.
摘要
确定了来自感染牛巴贝斯虫的红细胞裂解物的最佳凝胶过滤级分，用作酶联免疫吸附测定（ELISA）中的抗原，以诊断牛的牛巴贝斯虫感染。在所测试的四种酶标记物中，辣根过氧化物酶是最合适的。该测定法在单次感染后2至4年内检测抗体时既灵敏又特异。仅在一些感染边缘无浆体的牛的某些血清中出现假阳性反应。
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