用重组棒状体相关蛋白1抗原酶联免疫吸附试验检测牛巴贝斯虫特异性抗体在牛中的应用评价。
Evaluation of an enzyme-linked immunosorbent assay with recombinant rhoptry-associated protein 1 antigen against Babesia bovis for the detection of specific antibodies in cattle.
作者信息
Boonchit Suthisak, Xuan Xuenan, Yokoyama Naoaki, Goff Will L, Wagner Gale, Igarashi Ikuo
机构信息
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
出版信息
J Clin Microbiol. 2002 Oct;40(10):3771-5. doi: 10.1128/JCM.40.10.3771-3775.2002.
Abstract
The gene encoding Babesia bovis rhoptry-associated protein 1 (RAP-1) was used to develop an enzyme-linked immunosorbent assay (ELISA) to measure specific antibodies against B. bovis. The B. bovis RAP-1 gene was subcloned into a baculovirus transfer vector, and the RAP-1 protein was expressed in insect cells infected with a recombinant baculovirus. The recombinant B. bovis RAP-1 of 65 kDa was detected with anti-RAP-1 mouse serum by Western blotting, and this recombinant RAP-1 was used as an antigen in the ELISA. The ELISA was able to differentiate between B. bovis-infected sera and B. bigemina-infected sera or noninfected normal bovine sera. The results demonstrate that the recombinant RAP-1 expressed in insect cells might be a useful antigen for the detection of antibodies to B. bovis.
摘要
编码牛巴贝斯虫（Babesia bovis）棒状体相关蛋白1（RAP-1）的基因被用于开发一种酶联免疫吸附测定（ELISA），以检测针对牛巴贝斯虫的特异性抗体。牛巴贝斯虫RAP-1基因被亚克隆到杆状病毒转移载体中，并且RAP-1蛋白在感染重组杆状病毒的昆虫细胞中表达。通过蛋白质印迹法用抗RAP-1小鼠血清检测到了65 kDa的重组牛巴贝斯虫RAP-1，并且这种重组RAP-1被用作ELISA中的抗原。该ELISA能够区分牛巴贝斯虫感染血清与双芽巴贝斯虫（B. bigemina）感染血清或未感染的正常牛血清。结果表明，在昆虫细胞中表达的重组RAP-1可能是用于检测牛巴贝斯虫抗体的有用抗原。
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An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against Babesia bovis in cattle.一种用于检测牛抗牛巴贝斯虫抗体的酶联免疫吸附测定（ELISA）。
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