发育毒理学中的诱导多能干细胞（iPSC）

Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology.

作者信息

Easley Charles A

机构信息

Department of Environmental Health Science, University of Georgia College of Public Health, Athens, GA, USA.

出版信息

Methods Mol Biol. 2019;1965:19-34. doi: 10.1007/978-1-4939-9182-2_3.

DOI:10.1007/978-1-4939-9182-2_3

PMID:31069666

Abstract

The discovery of induced pluripotent stem cells (iPSCs) by Dr. Shinya Yamanaka and his team has opened up many avenues of research. This includes medical initiatives such as the Precision Medicine and Personalized Medicine initiatives to use patient-specific stem cells to guide medical professionals on the base courses of treatment for various disorders based on the patient's own genetic background, i.e., targeting the best treatment for the individual patient. However iPSC technology has greater potential than disease modeling and regenerative medicine therapies. In this chapter, we will outline how to culture and maintain human iPSCs, differentiate human iPSCs into neurons, and discuss how iPSCs can be utilized for developmental toxicology studies. Furthermore, this chapter will highlight a burgeoning field using iPSCs to examine personalized exposure risks.

摘要

山中伸弥博士及其团队对诱导多能干细胞（iPSC）的发现开辟了许多研究途径。这包括一些医学举措，如精准医学和个性化医学计划，即利用患者特异性干细胞，根据患者自身的基因背景，为医学专业人员在各种疾病的基础治疗方案上提供指导，也就是针对个体患者制定最佳治疗方案。然而，iPSC技术的潜力远不止于疾病建模和再生医学疗法。在本章中，我们将概述如何培养和维持人iPSC，如何将人iPSC分化为神经元，并讨论iPSC如何用于发育毒理学研究。此外，本章还将重点介绍一个新兴领域，即利用iPSC来检测个性化暴露风险。