Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
Department of Physiology and Pharmacology, The University of Western Ontario, London, Ontario, N6A 5C1, Canada.
BMC Vet Res. 2020 Dec 8;16(1):477. doi: 10.1186/s12917-020-02696-7.
Induced pluripotent stem cells (iPSCs) are undifferentiated stem cells characterized by the ability to differentiate into any cell type in the body. iPSCs are a relatively new and rapidly developing technology in many fields of biology, including developmental anatomy and physiology, pathology, and toxicology. These cells have great potential in research as they are self-renewing and pluripotent with minimal ethical concerns. Protocols for their production have been developed for many domestic animal species, which have since been used to further our knowledge in the progression and treatment of diseases. This research is valuable both for veterinary medicine as well as for the prospect of translation to human medicine. Safety, cost, and feasibility are potential barriers for this technology that must be considered before widespread clinical adoption. This review will analyze the literature pertaining to iPSCs derived from various domestic species with a focus on iPSC production and characterization, applications for tissue and disease research, and applications for disease treatment.
诱导多能干细胞（iPSCs）是一种未分化的干细胞，其特征是能够分化为体内任何细胞类型。iPSCs 是许多生物学领域（包括发育解剖学和生理学、病理学和毒理学）中相对较新且快速发展的技术。这些细胞在研究中具有很大的潜力，因为它们具有自我更新和多能性，并且伦理问题较少。已经为许多家畜物种开发了它们的生产方案，这些方案随后被用于进一步了解疾病的进展和治疗。这项研究对于兽医和人类医学的转化都具有重要价值。在广泛的临床应用之前，必须考虑该技术的安全性、成本和可行性等潜在障碍。本综述将分析源自各种家畜的 iPSCs 的文献，重点关注 iPSC 的生产和表征、组织和疾病研究的应用以及疾病治疗的应用。
