使用CRISPR-Cas9对斑马鱼进行基因组编辑：在发育毒理学中的应用

Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology.

作者信息

Warner Brendon K, Alder Jonathan K, Suli Arminda

机构信息

Department of Physiology and Developmental Biology, College of Life Sciences, Brigham Young University, Provo, UT, USA.

Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

出版信息

Methods Mol Biol. 2019;1965:235-250. doi: 10.1007/978-1-4939-9182-2_16.

DOI:10.1007/978-1-4939-9182-2_16

PMID:31069679

Abstract

Environment-gene interactions have a powerful impact on embryo development. The ability to precisely edit the genome makes it possible to address questions concerning the specific roles that genes or variants play in modulating the response to environmental challenges. In this chapter, we provide a simplified protocol using CRISPR-Cas9 ribonucleoproteins for genome editing in the zebrafish model organism. The genetic manipulation can then be coupled with chemical screens to identify and understand the mechanism behind toxicants or compounds that modulate development.

摘要

环境与基因的相互作用对胚胎发育有着强大的影响。精确编辑基因组的能力使得解决有关基因或变体在调节对环境挑战的反应中所起的特定作用的问题成为可能。在本章中，我们提供了一种使用CRISPR-Cas9核糖核蛋白在斑马鱼模式生物中进行基因组编辑的简化方案。然后可以将基因操作与化学筛选相结合，以识别和理解调节发育的有毒物质或化合物背后的机制。