Dubey Rituraj, Bhushan Ravi
Department of Chemistry, Badri Narayan Mukteshwar College Barhiya, Munger University, Bihar, India.
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India.
Methods Mol Biol. 2019;1985:35-44. doi: 10.1007/978-1-4939-9438-0_2.
Despite the fact that high-performance liquid chromatography is the predominant technique for analytical and preparative enantioseparations, chiral thin-layer chromatography (TLC) may represent an alternative, especially if fast analysis with simple equipment is required. This chapter describes several approaches in chiral TLC for the separation of amino acids and basic drugs using DL-selenomethionine and β-adrenergic drugs as examples. Analytical approaches include the impregnation of the adsorbent with a chiral selector using pre-coated as well as custom-prepared TLC plates and addition of the selector to the mobile phase directly as well as in the form of copper metal complex. (-)-Quinine and L-amino acids were used as chiral selectors in different manners for enantioseparations.
尽管高效液相色谱法是用于分析和制备对映体分离的主要技术，但手性薄层色谱法（TLC）可能是一种替代方法，特别是在需要使用简单设备进行快速分析的情况下。本章以DL-硒代蛋氨酸和β-肾上腺素能药物为例，介绍了手性TLC中分离氨基酸和碱性药物的几种方法。分析方法包括使用预涂以及定制制备的TLC板用手性选择剂浸渍吸附剂，以及直接将选择剂以铜金属配合物的形式添加到流动相中。（-）-奎宁和L-氨基酸以不同方式用作对映体分离的手性选择剂。
