Wang S C, Greenwald G S
Proc Soc Exp Biol Med. 1987 May;185(1):55-61. doi: 10.3181/00379727-185-42516.
Hamsters were injected sc at 1400 hr on proestrus with either 4 mg cycloheximide (which blocks ovulation but only transiently affects ovarian protein synthesis) or saline and killed at 2-hr intervals until 0400 hr on estrus. After cycloheximide, the first surge of FSH (at 1600 hr) was half the normal value and the second surge of FSH (beginning at 2200 hr) was eliminated. Control follicles at 1400 hr had approximately the same number of FSH and hCG receptors with about one-third as many PRL receptors. Down regulation of FSH and hCG receptors for control follicles occurred by 2400 hr while PRL receptors dropped abruptly 4 hr earlier. Compared to the 1400-hr control values, the maximal loss of FSH, LH, and PRL receptors was 40, 45, and 85%, respectively. Although cycloheximide tended to slightly delay the loss of FSH receptors at 2000-2200 hr it did not prevent the ultimate fall in FSH and hCG receptors; the loss of PRL receptors was accelerated by 4 hr. Cycloheximide prevented or delayed follicular growth, resumption of meiosis, and cumulus expansion. The altered proestrous profile of steroids after cycloheximide (prolonged follicular estradiol and reduced progesterone) is therefore not associated with drastic alterations in the number of FSH and hCG binding sites. On the other hand, PRL receptors represent fast turnover protein(s).
在动情前期的14:00给仓鼠皮下注射4毫克环己酰亚胺（其可阻断排卵，但仅短暂影响卵巢蛋白质合成）或生理盐水，并每隔2小时处死一批，直至动情期的04:00。注射环己酰亚胺后，促卵泡激素的第一次激增（16:00时）为正常值的一半，第二次激增（22:00开始）则消失。14:00时对照卵泡的促卵泡激素和人绒毛膜促性腺激素受体数量大致相同，催乳素受体数量约为其三分之一。对照卵泡的促卵泡激素和人绒毛膜促性腺激素受体在24:00时发生下调，而催乳素受体在早4小时时急剧下降。与14:00时的对照值相比，促卵泡激素、促黄体生成素和催乳素受体的最大损失分别为40%、45%和85%。尽管环己酰亚胺在20:00至22:00时倾向于略微延迟促卵泡激素受体的损失，但并未阻止促卵泡激素和人绒毛膜促性腺激素受体最终的下降；催乳素受体的损失提前了4小时加速。环己酰亚胺可阻止或延迟卵泡生长、减数分裂的恢复以及卵丘扩展。因此，环己酰亚胺处理后动情前期类固醇的变化（卵泡雌二醇延长和孕酮减少）与促卵泡激素和人绒毛膜促性腺激素结合位点数量的剧烈变化无关。另一方面，催乳素受体代表快速周转蛋白。
