a Department of Medicine, Faculty of Medicine, Qom Branch , Islamic Azad University , Qom , Iran.
b Materials and Nuclear Fuel Research School , Nuclear Science and Technology Research Institute , Tehran , Iran.
Pharm Biol. 2018 Dec;56(1):495-504. doi: 10.1080/13880209.2018.1510972.
Mentha longifolia L. (Lamiaceae), a traditional Iranian plant, possesses antimicrobial and antioxidant activities.
We investigated the potential protective effects of M. longifolia essential oils (E.Os) on caecal ligation and puncture (CLP) induced liver injury.
Wistar Albino rats (n = 50) were grouped as follows: (1) a laparotomy group (LAP); (2) a CLP group (CLP); (3) the treatment groups received orally the E.Os (50 and 100 mg/kg b.w) and indomethacin (2 mg/kg b.w) for 2 weeks. The oxidative stress parameters, liver enzymes and prostaglandin E2 (PGE2) level were measured in liver and plasma tissues. The liver was also harvested for the real time PCR of cyclooxygenase (COX-2) expression following histopathological examinations.
The results indicated that the CLP operation significantly increased lipid peroxidation (LP) [1.79-fold], myeloperoxidase (MPO) [2.76-fold], PGE2 [1.56-fold] besides plasma aspartate aminotransferase (AST) [2.4-fold] and alanine aminotransferase (ALT) activities [2.22-fold], while, markedly reduced glutathione (GSH) [0.63-fold] and ferric reducing ability of plasma (FRAP) levels [0.63-fold]. Even COX2 expression significantly increased in the CLP group as compared to the LAP group. Treatments of rats with the E.Os could return all the hepatic and plasma biomarkers to the normal levels. These results were further confirmed by pathological examination on liver indicating that E.Os could successfully improve the CLP-induced liver injuries.
Our findings suggest that E.Os is able to protect liver injuries against sepsis via modulating the oxidative stress parameters concomitant with the suppression of inflammatory reactions such as PGE2 and COX-2.
唇形科植物长叶薄荷（Lamiaceae），一种传统的伊朗植物，具有抗菌和抗氧化活性。
研究长叶薄荷精油（E.Os）对盲肠结扎穿刺（CLP）诱导的肝损伤的潜在保护作用。
将 Wistar 白化大鼠（n=50）分为以下几组：（1）剖腹手术组（LAP）；（2）CLP 组（CLP）；（3）治疗组分别给予 E.Os（50 和 100mg/kg b.w）和吲哚美辛（2mg/kg b.w）口服，共 2 周。在肝和血浆组织中测量氧化应激参数、肝酶和前列腺素 E2（PGE2）水平。还进行了实时聚合酶链反应（PCR）以检测环氧化酶（COX-2）的表达，并进行了组织病理学检查。
结果表明，CLP 手术显著增加了脂质过氧化（LP）[1.79 倍]、髓过氧化物酶（MPO）[2.76 倍]、PGE2[1.56 倍]，同时还增加了血浆天冬氨酸氨基转移酶（AST）[2.4 倍]和丙氨酸氨基转移酶（ALT）[2.22 倍]的活性，而还原型谷胱甘肽（GSH）[0.63 倍]和血浆铁还原能力（FRAP）水平[0.63 倍]则显著降低。甚至 COX2 的表达在 CLP 组中也明显高于 LAP 组。用 E.Os 治疗大鼠可使所有肝和血浆生物标志物恢复正常水平。这些结果通过对肝脏的病理检查进一步得到证实，表明 E.Os 能够成功地改善 CLP 诱导的肝损伤。
我们的研究结果表明，E.Os 能够通过调节氧化应激参数，同时抑制 PGE2 和 COX-2 等炎症反应，保护肝脏免受败血症的损伤。
