1 University of Minnesota, Department of Plant Pathology, Saint Paul, MN 55108.
2 USDA-ARS, National Germplasm Resources Laboratory, Beltsville, MD 20705.
Plant Dis. 2019 Jun;103(6):1391-1396. doi: 10.1094/PDIS-11-18-2112-RE. Epub 2019 May 8.
The genomic, biological, and serological characterization of tomato necrotic spot virus (ToNSV), a virus first described infecting tomato in California, was completed. The complete genomic sequence identified ToNSV as a new subgroup 1 ilarvirus distinct from the previously described tomato-infecting ilarviruses. We identified ToNSV in Indiana in 2017 and 2018 and in Ohio in 2018. The coat protein coding region of the isolates from California, Indiana, and Ohio have 94 to 98% identity, while the same isolates had 99% amino acid identity. ToNSV is serologically related to TSV, a subgroup 1 ilarvirus, and shows no serological relationship to ilarviruses in the other subgroups. In tomato, ToNSV caused symptoms of necrotic spots and flecks on leaves, necrotic streaking on stems, and necrotic spots and circular patterns on fruit resulting in a yield loss of 1 to 13%. These results indicate that ToNSV is a proposed new subgroup 1 ilarvirus causing a necrotic spotting disease of tomato observed in California, Indiana, and Ohio.
番茄坏死斑点病毒（ToNSV）的基因组、生物学和血清学特征已被完成。该病毒最初被描述为感染加利福尼亚州的番茄，其完整的基因组序列将其确定为一个新的亚组 1 杆状病毒，与之前描述的感染番茄的杆状病毒不同。我们于 2017 年和 2018 年在印第安纳州以及 2018 年在俄亥俄州发现了 ToNSV。来自加利福尼亚、印第安纳和俄亥俄的分离物的外壳蛋白编码区具有 94%至 98%的同一性，而相同的分离物具有 99%的氨基酸同一性。ToNSV 在血清学上与 TSV 相关，后者是一个亚组 1 杆状病毒，与其他亚组中的杆状病毒没有血清学关系。在番茄中，ToNSV 引起叶片坏死斑点和斑纹、茎部坏死条纹以及果实坏死斑点和圆形图案的症状，导致 1%至 13%的产量损失。这些结果表明，ToNSV 是一种新提出的亚组 1 杆状病毒，可引起加利福尼亚、印第安纳和俄亥俄州观察到的番茄坏死斑点病。
