Preclik G, Stange E F, Arnold D, Kraft O, Schneider A, Ditschuneit H
Prostaglandins. 1987 Mar;33(3):459-77. doi: 10.1016/0090-6980(87)90026-8.
In the present study we systematically investigated the synthesis of prostaglandins in the mucosa and the muscle layer along the length of the rabbit gut. Homogenates of mucosa and muscle layer were incubated with (14C)-labelled arachidonic acid, and prostaglandin formation was determined using thin-layer chromatography. With respect to total prostaglandin synthesis the highest values in the mucosa were measured in fundus, antrum and colon, whereas the prostaglandin synthesis in the muscle layer was maximal in the small bowel, particularly the ileum. In the mucosa, the prostaglandins E2 and F2a predominated, and there were minor differences along the gastrointestinal tract. In the muscle layer of the stomach, high amounts of 6-keto prostaglandin F1a, the stable degradation product of prostacyclin were produced, while small and large bowel homogenates synthesized mostly F2a. Consistently the prostaglandins A2/B2 were a major product in most locations. In addition, PG E2 catabolism to 15-keto PG E2 and 13,14-dihydro-15-keto PG E2 in the absence of NAD was slow. No significant changes in total prostaglandin synthesis and prostaglandin profile were detected between 24 hrs fasted and normally fed rabbits at any part of the gastrointestinal tract.
在本研究中，我们系统地研究了兔肠道全长黏膜和肌层中前列腺素的合成。将黏膜和肌层的匀浆与（14C）标记的花生四烯酸一起孵育，并使用薄层色谱法测定前列腺素的形成。就总前列腺素合成而言，黏膜中最高值出现在胃底、胃窦和结肠，而肌层中前列腺素合成在小肠尤其是回肠中最高。在黏膜中，前列腺素E2和F2a占主导，并且在胃肠道中存在微小差异。在胃的肌层中，产生了大量的6-酮前列腺素F1a，即前列环素的稳定降解产物，而小肠和大肠匀浆主要合成F2a。一致地，前列腺素A2/B2在大多数部位是主要产物。此外，在没有NAD的情况下，PG E2分解代谢为15-酮PG E2和13,14-二氢-15-酮PG E2的速度较慢。在禁食24小时的兔子和正常喂食的兔子之间，在胃肠道的任何部位均未检测到总前列腺素合成和前列腺素谱的显著变化。
